Linked Life Data

12498090

Source:http://linkedlifedata.com/resource/pubmed/id/12498090

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2002-12-24
pubmed:abstractText
The results of NSE purification procedure, as well as hybridoma technology of anti NSE monoclonal antibodies synthesis are presented. The employment of this procedure yielded highly purified NSE preparation. The immunization of BALB/C mice with NSE preparation led to sensitization of the immunocompetent cells, which could form hybridomes, producing the anti-NSE monoclonal antibodies, after the confluence with myeloma cells Sp 2/0-Ag 14. The ELISA test-system for NSE analysis was developed on the basis of highly purified NSE preparation and monoclonal anti-NSE antibodies. This system was characterized by high specificity, accuracy and reliability. This system may be recommended for analysis of blood-brain barrier functions in the neurological and psychiatric diseases.
pubmed:language
rus
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0042-8809
pubmed:author
pubmed:issnType
Print
pubmed:volume
48
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
477-84
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:articleTitle
[Monoclonal anti-NSE-antibodies: purification, characterization, and immunoenzyme analysis].
pubmed:affiliation
Serbsky National Research Centre for Social and Forensic Psychiatry, Moscow, Russia.
pubmed:publicationType
Journal Article, English Abstract