Source:http://linkedlifedata.com/resource/pubmed/id/12496384
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2002-12-23
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| pubmed:abstractText |
Using high sensitivity fluorescence imaging with shutter speeds approximately 600,000 times faster than those of video frames, we have characterized Ca2+ waves within cells in exquisite detail to reveal Ca2+ signaling routes. Polarized neutrophils exhibited a counterclockwise rotating ryanodine-sensitive juxtamembrane Ca2+ wave during temporal calcium spikes. During stimulation with fMLP, a chemotactic factor, two Ca2+ waves traveling in opposite directions around the perimeter of the cell emanated from sites of stimulation (the clockwise wave is verapamil sensitive). Phagocytosed targets exhibit counterclockwise Ca2+ waves traveling about their periphery originating from the plasma membrane. This study: 1) outlines the technology to observe Ca2+ signaling circuitry within small living cells; 2) shows that extracellular spatial information in the form of a chemotactic factor gradient is transduced into intracellular chemical patterns, which provides fresh insights in signaling; 3) suggests that a line of communication exits between the cell surface and phagosomes; and 4) suggests that spatiotemporal Ca2+ patterns contribute to drug actions.
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| pubmed:grant | |
| pubmed:commentsCorrections | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Cations, Divalent,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Indoles,
http://linkedlifedata.com/resource/pubmed/chemical/N-Formylmethionine...,
http://linkedlifedata.com/resource/pubmed/chemical/indo-1
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jan
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| pubmed:issn |
0022-1767
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
1
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| pubmed:volume |
170
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
64-72
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| pubmed:dateRevised |
2009-3-30
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| pubmed:meshHeading |
pubmed-meshheading:12496384-Animals,
pubmed-meshheading:12496384-Calcium,
pubmed-meshheading:12496384-Calcium Signaling,
pubmed-meshheading:12496384-Cations, Divalent,
pubmed-meshheading:12496384-Cell Polarity,
pubmed-meshheading:12496384-Chemotaxis, Leukocyte,
pubmed-meshheading:12496384-Erythrocytes,
pubmed-meshheading:12496384-Fluorescent Dyes,
pubmed-meshheading:12496384-Humans,
pubmed-meshheading:12496384-Indoles,
pubmed-meshheading:12496384-Intracellular Fluid,
pubmed-meshheading:12496384-Microscopy, Fluorescence,
pubmed-meshheading:12496384-Microspectrophotometry,
pubmed-meshheading:12496384-N-Formylmethionine Leucyl-Phenylalanine,
pubmed-meshheading:12496384-Neutrophil Activation,
pubmed-meshheading:12496384-Neutrophils,
pubmed-meshheading:12496384-Phagocytosis,
pubmed-meshheading:12496384-Phagosomes,
pubmed-meshheading:12496384-Sheep
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| pubmed:year |
2003
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| pubmed:articleTitle |
Intracellular calcium waves accompany neutrophil polarization, formylmethionylleucylphenylalanine stimulation, and phagocytosis: a high speed microscopy study.
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| pubmed:affiliation |
Department of Biological Sciences, Wayne State University, Detroit, MI 48202, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Retracted Publication
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