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rdf:type |
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lifeskim:mentions |
umls-concept:C0015259,
umls-concept:C0026845,
umls-concept:C0034693,
umls-concept:C0034721,
umls-concept:C0037083,
umls-concept:C0051028,
umls-concept:C0456205,
umls-concept:C0599781,
umls-concept:C1280500,
umls-concept:C1550605,
umls-concept:C1710082,
umls-concept:C1995013
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pubmed:issue |
4
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pubmed:dateCreated |
2003-3-10
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pubmed:abstractText |
Physical activity is known to increase insulin action in skeletal muscle, and data have indicated that 5'-AMP-activated protein kinase (AMPK) is involved in the molecular mechanisms behind this beneficial effect. 5-Aminoimidazole-4-carboxamide-1-beta-d-ribofuranoside (AICAR) can be used as a pharmacological tool to repetitively activate AMPK, and the objective of this study was to explore whether the increase in insulin-stimulated glucose uptake after either long-term exercise or chronic AICAR administration was followed by fiber-type-specific changes in insulin signaling and/or changes in GLUT-4 expression. Wistar rats were allocated into three groups: an exercise group trained on treadmill for 5 days, an AICAR group exposed to daily subcutaneous injections of AICAR, and a sedentary control group. AMPK activity, insulin-stimulated glucose transport, insulin signaling, and GLUT-4 expression were determined in muscles characterized by different fiber type compositions. Both exercised and AICAR-injected animals displayed a fiber-type-specific increase in glucose transport with the most marked increase in muscles with a high content of type IIb fibers. This increase was accompanied by a concomitant increase in GLUT-4 expression. Insulin signaling as assessed by phosphatidylinositol 3-kinase and PKB/Akt activity was enhanced only after AICAR administration and in a non-fiber-type-specific manner. In conclusion, chronic AICAR administration and long-term exercise both improve insulin-stimulated glucose transport in skeletal muscle in a fiber-type-specific way, and this is associated with an increase in GLUT-4 content.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/3'-O-methylguanosine,
http://linkedlifedata.com/resource/pubmed/chemical/AICA ribonucleotide,
http://linkedlifedata.com/resource/pubmed/chemical/AMP-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Akt1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Aminoimidazole Carboxamide,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose Transporter Type 4,
http://linkedlifedata.com/resource/pubmed/chemical/Guanosine,
http://linkedlifedata.com/resource/pubmed/chemical/Hypoglycemic Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin Receptor Substrate Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Intracellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Irs1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Irs2 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Monosaccharide Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Multienzyme Complexes,
http://linkedlifedata.com/resource/pubmed/chemical/Muscle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol 3-Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-akt,
http://linkedlifedata.com/resource/pubmed/chemical/Ribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Slc2a4 protein, rat
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
8750-7587
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
94
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1373-9
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:12496137-AMP-Activated Protein Kinases,
pubmed-meshheading:12496137-Aminoimidazole Carboxamide,
pubmed-meshheading:12496137-Animals,
pubmed-meshheading:12496137-Biological Transport,
pubmed-meshheading:12496137-Glucose,
pubmed-meshheading:12496137-Glucose Transporter Type 4,
pubmed-meshheading:12496137-Guanosine,
pubmed-meshheading:12496137-Hypoglycemic Agents,
pubmed-meshheading:12496137-Immunoblotting,
pubmed-meshheading:12496137-Insulin,
pubmed-meshheading:12496137-Insulin Receptor Substrate Proteins,
pubmed-meshheading:12496137-Intracellular Signaling Peptides and Proteins,
pubmed-meshheading:12496137-Male,
pubmed-meshheading:12496137-Monosaccharide Transport Proteins,
pubmed-meshheading:12496137-Motor Activity,
pubmed-meshheading:12496137-Multienzyme Complexes,
pubmed-meshheading:12496137-Muscle, Skeletal,
pubmed-meshheading:12496137-Muscle Proteins,
pubmed-meshheading:12496137-Phosphatidylinositol 3-Kinases,
pubmed-meshheading:12496137-Phosphoproteins,
pubmed-meshheading:12496137-Phosphorylation,
pubmed-meshheading:12496137-Protein-Serine-Threonine Kinases,
pubmed-meshheading:12496137-Proto-Oncogene Proteins,
pubmed-meshheading:12496137-Proto-Oncogene Proteins c-akt,
pubmed-meshheading:12496137-Rats,
pubmed-meshheading:12496137-Rats, Wistar,
pubmed-meshheading:12496137-Ribonucleotides,
pubmed-meshheading:12496137-Signal Transduction
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pubmed:year |
2003
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pubmed:articleTitle |
Effects of AICAR and exercise on insulin-stimulated glucose uptake, signaling, and GLUT-4 content in rat muscles.
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pubmed:affiliation |
Medical Research Laboratory and Medical Department M (Endocrinology and Diabetes), Aarhus University Hospital, Aarhus Kommunehospital, University of Aarhus, DK-8000 Aarhus C, Denmark.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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