Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1976-4-27
pubmed:abstractText
Antigen (Ag)-antibody (Ab) aggregates prepared with several different antigens are solubilized by fresh serum at 37 degrees C (complex-release activity of serum or CRA). The rate of solubilization varies in different systems and is strongly influenced by the affinity of Ab for the Ag in the immune precipitate. With a given Ag-Ab precipitate, the maximum amount of complex that can be solubilized by individual sera is independent of the initial concentration of complexes and cannot be increased by prolonged incubation. CRA occurs in the absence of C2 and C4, but not in the absence of C3 and factor B of the properdin pathway. Addition of C2 to C2-deficient serum or C4 to C4-deficient serum enhances CRA. Solubilization does not involve extensive degradation of the complexed antibody, as might be detected by acrylamide gel electrophoresis of released antibody after reduction and alkylation to separate H and L chains. Immune precipitates can also be solubilized by incubation with monovalent fragments (Fab or Fab') of antibodies against determinants of the Ab molecules in the immune precipitate. In contrast, F(ab')2 fragments decrease the solubility of the immune precipitates. In view of these findings, we propose that CRA is mediated by the binding of functionally monovalent C fragments (C3 and C4) onto Ab molecules in the precipitates.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1249522-1054824, http://linkedlifedata.com/resource/pubmed/commentcorrection/1249522-1117137, http://linkedlifedata.com/resource/pubmed/commentcorrection/1249522-1159084, http://linkedlifedata.com/resource/pubmed/commentcorrection/1249522-13580234, http://linkedlifedata.com/resource/pubmed/commentcorrection/1249522-14097352, http://linkedlifedata.com/resource/pubmed/commentcorrection/1249522-14280442, http://linkedlifedata.com/resource/pubmed/commentcorrection/1249522-14434282, http://linkedlifedata.com/resource/pubmed/commentcorrection/1249522-19870380, http://linkedlifedata.com/resource/pubmed/commentcorrection/1249522-4098210, http://linkedlifedata.com/resource/pubmed/commentcorrection/1249522-4111773, http://linkedlifedata.com/resource/pubmed/commentcorrection/1249522-4159289, http://linkedlifedata.com/resource/pubmed/commentcorrection/1249522-4160044, http://linkedlifedata.com/resource/pubmed/commentcorrection/1249522-4193274, http://linkedlifedata.com/resource/pubmed/commentcorrection/1249522-4386453, http://linkedlifedata.com/resource/pubmed/commentcorrection/1249522-4541628, http://linkedlifedata.com/resource/pubmed/commentcorrection/1249522-4542253, http://linkedlifedata.com/resource/pubmed/commentcorrection/1249522-4845664, http://linkedlifedata.com/resource/pubmed/commentcorrection/1249522-4861258, http://linkedlifedata.com/resource/pubmed/commentcorrection/1249522-4953701, http://linkedlifedata.com/resource/pubmed/commentcorrection/1249522-4971842, http://linkedlifedata.com/resource/pubmed/commentcorrection/1249522-5817170
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0022-1007
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
143
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
615-30
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1976
pubmed:articleTitle
Studies on the mechanism of solubilization of immune precipitates by serum.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.