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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2002-12-19
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pubmed:abstractText |
We tested the hypothesis that inhibition of Epstein-Barr virus (EBV) reactivation is controlled in part by nitric oxide (NO) generated from L-arginine (Arg). The spontaneous reactivation of EBV in the Burkitt's lymphoma (BL) cell line P3HR-1 was inhibited when the cells were cultured in L-Arg-supplemented medium. The expression of EBV early antigen (EA), immediate-early BZLF1 mRNA and the protein ZEBRA, and production of infectious virus were reduced by L-Arg supplementation in a dose-dependent manner. We demonstrated that inducible NO synthase (iNOS) mRNA was constitutively expressed in P3HR-1 cells, as quantitated by the reverse transcription-polymerase chain reaction. L-Arg supplementation enhanced iNOS and NOx expression in the cells. A specific NOS inhibitor, NG-monomethyl-L-Arg enhanced the expression of ZEBRA and early BMRF1 protein EA-D in the cells. L-Arg supplementation also inhibited the spontaneous EBV reactivation in another BL cell line EB1 and a B lymphoblastoid cell line OB. These results indicated that L-Arg induces iNOS and generates NO, which inhibits EBV reactivation in EBV-positive cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Arginine,
http://linkedlifedata.com/resource/pubmed/chemical/BRLF1 protein, Human herpesvirus 4,
http://linkedlifedata.com/resource/pubmed/chemical/BZLF1 protein, Herpesvirus 4, Human,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Immediate-Early Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/NOS2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase Type II,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/omega-N-Methylarginine
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0042-6822
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
5
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pubmed:volume |
304
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
114-24
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pubmed:dateRevised |
2011-10-27
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pubmed:meshHeading |
pubmed-meshheading:12490409-Arginine,
pubmed-meshheading:12490409-Blotting, Northern,
pubmed-meshheading:12490409-Blotting, Western,
pubmed-meshheading:12490409-Cell Division,
pubmed-meshheading:12490409-Cells, Cultured,
pubmed-meshheading:12490409-DNA-Binding Proteins,
pubmed-meshheading:12490409-Enzyme Inhibitors,
pubmed-meshheading:12490409-Gene Expression,
pubmed-meshheading:12490409-Herpesvirus 4, Human,
pubmed-meshheading:12490409-Humans,
pubmed-meshheading:12490409-Immediate-Early Proteins,
pubmed-meshheading:12490409-Nitric Oxide,
pubmed-meshheading:12490409-Nitric Oxide Synthase,
pubmed-meshheading:12490409-Nitric Oxide Synthase Type II,
pubmed-meshheading:12490409-Trans-Activators,
pubmed-meshheading:12490409-Viral Proteins,
pubmed-meshheading:12490409-omega-N-Methylarginine
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pubmed:year |
2002
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pubmed:articleTitle |
Down-regulation of spontaneous Epstein-Barr virus reactivation in the P3HR-1 cell line by L-arginine.
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pubmed:affiliation |
Department of Biosignaling, School of Life Science, Faculty of Medicine, Tottori University, Yonago, 683-8503, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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