Linked Life Data

12489177

Source:http://linkedlifedata.com/resource/pubmed/id/12489177

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2-3
pubmed:dateCreated
2002-12-19
pubmed:abstractText
Two highly expressed noncollagenous proteins associated with dentin mineralization, dentin sialoprotein (DSP) and phosphophoryn (PP), are encoded by a single DSP-PP transcript. To better understand how DSP-PP transcripts are regulated, we have determined the DSP-PP transcription start site, sequenced its 5' flanking region, and analyzed the transcriptional activity of the gene promoter out to -1615 bp. Comparison of the rat cDNA sequence with the mouse, rat and human genes clearly indicates high sequence conservation within the DSP-PP 5' flanking region, implicating the possible presence of highly conserved gene regulatory cis elements. Among a number of conserved transcription sites identified in the 5' flanking region, we demonstrate that the conserved Y box sequence (ATTGG) can specifically bind nuclear extracts from mouse MDPC23 cells. This sequence (located within the -57 bp/-52 bp 5' flanking region) therefore likely represents one DSP-PP transcriptional regulatory sequence.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0300-8207
pubmed:author
pubmed:issnType
Print
pubmed:volume
43
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
331-7
pubmed:dateRevised
2010-2-5
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
The conservation and regulation of rat DSP-PP gene.
pubmed:affiliation
Department of Cariology, Restorative Sciences, and Endodontics, University of Michigan School of Dentistry, 1011 North University, Ann Arbor, MI 48109-1078, USA. helenar@umich.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.