Linked Life Data

12487728

Source:http://linkedlifedata.com/resource/pubmed/id/12487728

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2002-12-18
pubmed:abstractText
1. Slices of human and rat liver were cryopreserved in 18% dimethyl sulphoxide (DMSO) and subsequently stored in liquid nitrogen for periods up to as long as 6 months. After thawing, the metabolism of testosterone to hydroxylated products and conjugation of 7-hydroxycoumarin were investigated. 2. Rat liver slices stored in liquid nitrogen for 6 months exhibited rates of formation of 7alpha-, 6beta- 16alpha- and 2alpha-hydroxytestosterone, and of androstenedione that did not differ significantly from those observed with fresh slices. 3. No formation of 2alpha-hydroxytestosterone was detected with slices of human liver. However, in contrast with the rat, human slices produced 2beta-hydroxytestosterone. The rates of formation of 7alpha-, 6beta-, 16alpha- and 2beta-hydroxytestosterone and of androstenedione by human liver slices after 6 months of storage in liquid nitrogen were 82, 71, 236, 66 and 92%, respectively, of the corresponding rates by fresh slices. 4. The rates of sulphation and glucuronidation of 7-hydroxycoumarin by slices from rat liver were 97 and 119%, respectively, of the corresponding fresh values after 6 months of storage in liquid nitrogen. 5. 7-Hydroxycoumarin glucuronidation by human liver slices was 53% of the corresponding fresh values after 6 months of storage. However, human slices showed little or no capacity to conjugate 7-hydroxycoumarin with sulphate. 6. It was demonstrated that slices of both human and rat liver can be cryopreserved and stored in liquid nitrogen for at least 6 months without major changes in their rates of metabolism of testosterone to its hydroxylated products and of 7-hydroxycoumarin conjugation. These findings further emphasize that cryopreservation of liver slices can be an effective tool in the use of biological material of limited availability.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0049-8254
pubmed:author
pubmed:issnType
Print
pubmed:volume
32
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
985-96
pubmed:dateRevised
2005-11-17
pubmed:meshHeading
pubmed-meshheading:12487728-Androstenedione, pubmed-meshheading:12487728-Animals, pubmed-meshheading:12487728-Chromatography, High Pressure Liquid, pubmed-meshheading:12487728-Coloring Agents, pubmed-meshheading:12487728-Cryopreservation, pubmed-meshheading:12487728-Dimethyl Sulfoxide, pubmed-meshheading:12487728-Freezing, pubmed-meshheading:12487728-Humans, pubmed-meshheading:12487728-Liver, pubmed-meshheading:12487728-Male, pubmed-meshheading:12487728-Nitrogen, pubmed-meshheading:12487728-Oxazines, pubmed-meshheading:12487728-Rats, pubmed-meshheading:12487728-Rats, Sprague-Dawley, pubmed-meshheading:12487728-Specimen Handling, pubmed-meshheading:12487728-Testosterone, pubmed-meshheading:12487728-Time Factors, pubmed-meshheading:12487728-Umbelliferones, pubmed-meshheading:12487728-Xanthenes
pubmed:year
2002
pubmed:articleTitle
Characterization of testosterone metabolism and 7-hydroxycoumarin conjugation by rat and human liver slices after storage in liquid nitrogen for 1 h up to 6 months.
pubmed:affiliation
Research DMPK, AstraZeneca R&D Södertälje, S-151 85 Södertälje, Sweden.
pubmed:publicationType
Journal Article