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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2-3
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pubmed:dateCreated |
2002-12-17
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pubmed:abstractText |
A choline (CHO) biosensor based on the determination of H(2)O(2) generated at the electrode surface by the enzyme choline oxidase (CHOx) was developed. The biosensor consisted of CHOx retained onto a horseradish peroxidase (HRP) immobilized solid carbon paste electrode (sCPE). The HRPsCPE contained the molecule phenothiazine as redox mediator and CHOx was physically retained on the electrode surface using a dialysis membrane. Several parameters have been studied such as, mediator amount, influence of applied potential, etc. The CHO measurements were performed in 0.1 M phosphate buffer, pH 7.4. Amperometric detection of CHO was realized at an applied potential of 0.0 mV vs Ag/AgCl. The response is linear over the concentration range 5.0x10(-7)-7.0x10(-5) M, with a detection limit of 1.0x10(-7) M. This biosensor was used to detect choline released from phosphatidylcholine (PC) by phospholipase D (PLD) in isolated rat salivary gland cells stimulated by a purinergic agonist (ATP).
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0956-5663
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
18
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
185-91
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pubmed:dateRevised |
2009-7-14
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pubmed:meshHeading |
pubmed-meshheading:12485764-Adenosine Triphosphate,
pubmed-meshheading:12485764-Alcohol Oxidoreductases,
pubmed-meshheading:12485764-Animals,
pubmed-meshheading:12485764-Biosensing Techniques,
pubmed-meshheading:12485764-Choline,
pubmed-meshheading:12485764-Coated Materials, Biocompatible,
pubmed-meshheading:12485764-Electrochemistry,
pubmed-meshheading:12485764-Membranes, Artificial,
pubmed-meshheading:12485764-Phosphatidylcholines,
pubmed-meshheading:12485764-Phospholipases,
pubmed-meshheading:12485764-Rats,
pubmed-meshheading:12485764-Reproducibility of Results,
pubmed-meshheading:12485764-Sensitivity and Specificity,
pubmed-meshheading:12485764-Submandibular Gland,
pubmed-meshheading:12485764-Temperature
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pubmed:year |
2003
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pubmed:articleTitle |
Amperometric determination of choline released from rat submandibular gland acinar cells using a choline oxidase biosensor.
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pubmed:affiliation |
Service de chimie analytique instrumentale et de bioélectrochimie, Université Libre de Bruxelles, Institut de Pharmacie, Campus Plaine, 1050 Bruxelles, Belgium.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't,
Evaluation Studies,
Validation Studies
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