| pubmed-article:12485757 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12485757 | lifeskim:mentions | umls-concept:C0029144 | lld:lifeskim |
| pubmed-article:12485757 | lifeskim:mentions | umls-concept:C0041942 | lld:lifeskim |
| pubmed-article:12485757 | lifeskim:mentions | umls-concept:C0184511 | lld:lifeskim |
| pubmed-article:12485757 | lifeskim:mentions | umls-concept:C0034746 | lld:lifeskim |
| pubmed-article:12485757 | lifeskim:mentions | umls-concept:C0600364 | lld:lifeskim |
| pubmed-article:12485757 | pubmed:issue | 2-3 | lld:pubmed |
| pubmed-article:12485757 | pubmed:dateCreated | 2002-12-17 | lld:pubmed |
| pubmed-article:12485757 | pubmed:abstractText | An optical biosensor for urea measurements was developed. The operation of the sensor is based on the well-known urease enzyme-catalyzed hydrolysis of urea. The ammonium ions liberated in the reaction are detected with an ion selective optode membrane containing nonactin as ion selective ionophore and ETH 5294 chromoionophore in a thin (1 microm) plasticized poly(vinylchloride) film. The basic sensing element was home made of a microscope glass slide, a HeNe laser light source, photodiode light detector and light in coupling, de-coupling elements. The transducer membrane and the enzyme containing reaction layer were sandwich-cast with spin coating onto the surface of the sensing slide. The attenuation of the laser light propagating inside the glass wave-guide was used as signal for urea measurements. With this arrangement membranes provided good sensitivity (0.05 absorption unit when going from 0.1 to 1 mM urea) and short (16-20 s) response time. Taking advantage on the improved response time, flow injection urea measurements were made in the 0.01-2 mM concentration range. Thirty sample/hour analysis-rate, good peak-to-peak reproducibility (RSD=0.02) and recovery (95-104%) was achieved with buffer diluted urea solutions. Applications for the analysis of real samples are planned to do in the future. | lld:pubmed |
| pubmed-article:12485757 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12485757 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12485757 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12485757 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12485757 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12485757 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12485757 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12485757 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12485757 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12485757 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12485757 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12485757 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12485757 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12485757 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:12485757 | pubmed:issn | 0956-5663 | lld:pubmed |
| pubmed-article:12485757 | pubmed:author | pubmed-author:NagyGézaG | lld:pubmed |
| pubmed-article:12485757 | pubmed:author | pubmed-author:KovácsBarnaB | lld:pubmed |
| pubmed-article:12485757 | pubmed:author | pubmed-author:DombiRolandR | lld:pubmed |
| pubmed-article:12485757 | pubmed:author | pubmed-author:TóthKláraK | lld:pubmed |
| pubmed-article:12485757 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12485757 | pubmed:volume | 18 | lld:pubmed |
| pubmed-article:12485757 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12485757 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12485757 | pubmed:pagination | 111-8 | lld:pubmed |
| pubmed-article:12485757 | pubmed:dateRevised | 2009-7-14 | lld:pubmed |
| pubmed-article:12485757 | pubmed:meshHeading | pubmed-meshheading:12485757... | lld:pubmed |
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| pubmed-article:12485757 | pubmed:meshHeading | pubmed-meshheading:12485757... | lld:pubmed |
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| pubmed-article:12485757 | pubmed:meshHeading | pubmed-meshheading:12485757... | lld:pubmed |
| pubmed-article:12485757 | pubmed:meshHeading | pubmed-meshheading:12485757... | lld:pubmed |
| pubmed-article:12485757 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:12485757 | pubmed:articleTitle | Optical biosensor for urea with improved response time. | lld:pubmed |
| pubmed-article:12485757 | pubmed:affiliation | Department for General and Physical Chemistry, University of Pécs, 7624 Pécs, Ifjúság u. 6, Hungary. | lld:pubmed |
| pubmed-article:12485757 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12485757 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
