Linked Life Data

12485757

Source:http://linkedlifedata.com/resource/pubmed/id/12485757

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2-3
pubmed:dateCreated
2002-12-17
pubmed:abstractText
An optical biosensor for urea measurements was developed. The operation of the sensor is based on the well-known urease enzyme-catalyzed hydrolysis of urea. The ammonium ions liberated in the reaction are detected with an ion selective optode membrane containing nonactin as ion selective ionophore and ETH 5294 chromoionophore in a thin (1 microm) plasticized poly(vinylchloride) film. The basic sensing element was home made of a microscope glass slide, a HeNe laser light source, photodiode light detector and light in coupling, de-coupling elements. The transducer membrane and the enzyme containing reaction layer were sandwich-cast with spin coating onto the surface of the sensing slide. The attenuation of the laser light propagating inside the glass wave-guide was used as signal for urea measurements. With this arrangement membranes provided good sensitivity (0.05 absorption unit when going from 0.1 to 1 mM urea) and short (16-20 s) response time. Taking advantage on the improved response time, flow injection urea measurements were made in the 0.01-2 mM concentration range. Thirty sample/hour analysis-rate, good peak-to-peak reproducibility (RSD=0.02) and recovery (95-104%) was achieved with buffer diluted urea solutions. Applications for the analysis of real samples are planned to do in the future.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0956-5663
pubmed:author
pubmed:issnType
Print
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
111-8
pubmed:dateRevised
2009-7-14
pubmed:meshHeading
pubmed-meshheading:12485757-Biosensing Techniques, pubmed-meshheading:12485757-Calibration, pubmed-meshheading:12485757-Diamines, pubmed-meshheading:12485757-Enzymes, Immobilized, pubmed-meshheading:12485757-Equipment Design, pubmed-meshheading:12485757-Equipment Failure Analysis, pubmed-meshheading:12485757-Flow Injection Analysis, pubmed-meshheading:12485757-Macrolides, pubmed-meshheading:12485757-Membranes, Artificial, pubmed-meshheading:12485757-Microchemistry, pubmed-meshheading:12485757-Optics and Photonics, pubmed-meshheading:12485757-Oxazines, pubmed-meshheading:12485757-Quality Control, pubmed-meshheading:12485757-Reproducibility of Results, pubmed-meshheading:12485757-Sensitivity and Specificity, pubmed-meshheading:12485757-Spectrometry, Fluorescence, pubmed-meshheading:12485757-Urea
pubmed:year
2003
pubmed:articleTitle
Optical biosensor for urea with improved response time.
pubmed:affiliation
Department for General and Physical Chemistry, University of Pécs, 7624 Pécs, Ifjúság u. 6, Hungary.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't