Linked Life Data

12481932

Source:http://linkedlifedata.com/resource/pubmed/id/12481932

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2002-12-16
pubmed:abstractText
We have examined the influence of innervation on the expression of different isoforms of sarco(endo)plasmic reticulum Ca(2+)-ATPase (SERCA) in regenerating rat slow twitch muscle. The process of degeneration/regeneration was induced by injection of bupivacaine into rat soleus muscle under four different conditions: (1) in the presence of intact motor nerves, (2) after surgical denervation, (3) with nerve impulse conduction blocked by the Na(+)-channel blocker tetrodotoxin (TTX), and (4) with the axoplasmic flow blocked by vinblastine. Expression of SERCA isoforms was visualized by immunohistochemical and Western blot analysis. In regenerating innervated muscle, SERCA1, the isoform normally expressed in fast twitch fibres, was present after 5 days and was then progressively replaced by SERCA2, the isoform typical of slow twitch fibres. The maximum Ca(2+) uptake, measured in single skinned fibres regenerating for 10-21 days, was similar to that of slow adult fibres and significantly lower than that of fast adult fibres. Denervation or TTX treatment prevented the expression of the SERCA2 isoform. Conversely, vinblastine did not affect the expression of SERCA isoforms. These data indicate that nerve impulses play a determinant role in the expression of the SERCA2 isoform.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0958-0670
pubmed:author
pubmed:issnType
Print
pubmed:volume
87
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
575-83
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:12481932-Anesthetics, Local, pubmed-meshheading:12481932-Animals, pubmed-meshheading:12481932-Antineoplastic Agents, Phytogenic, pubmed-meshheading:12481932-Axonal Transport, pubmed-meshheading:12481932-Calcium, pubmed-meshheading:12481932-Calcium-Transporting ATPases, pubmed-meshheading:12481932-Motor Neurons, pubmed-meshheading:12481932-Muscle, Skeletal, pubmed-meshheading:12481932-Muscle Denervation, pubmed-meshheading:12481932-Muscle Fibers, Slow-Twitch, pubmed-meshheading:12481932-Rats, pubmed-meshheading:12481932-Rats, Wistar, pubmed-meshheading:12481932-Regeneration, pubmed-meshheading:12481932-Sarcoplasmic Reticulum Calcium-Transporting ATPases, pubmed-meshheading:12481932-Sciatic Nerve, pubmed-meshheading:12481932-Tetrodotoxin, pubmed-meshheading:12481932-Vinblastine
pubmed:year
2002
pubmed:articleTitle
Expression of sarco(endo)plasmic reticulum Ca(2+)-ATPase slow (SERCA2) isoform in regenerating rat soleus skeletal muscle depends on nerve impulses.
pubmed:affiliation
Dipartimento di Anatomia e Fisiologia Umana, Università di Padova, Via Marzolo 3, 35131 Padova, Italy.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't