Linked Life Data

12480131

Source:http://linkedlifedata.com/resource/pubmed/id/12480131

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2002-12-13
pubmed:abstractText
Elevating cortical serotonin (5-HT) in rats with clorgyline, a monoamine oxidase A (MAO(A)) inhibitor, from postnatal day (P-0) to P-6 delays the organization of thalamocortical afferent fibers into a vibrissae-related pattern in the somatosensory cortex (S-I). Despite continued elevation of cortical 5-HT through P-8, the thalamocortical fibers do form, albeit with some delay, a characteristic vibrissae pattern of barrels in layer IV of S-I by P-8. The growth-associated protein, GAP-43, is transiently expressed in developing S-I cortex of normal rats in a vibrissae related pattern until P-7. After P-7, GAP-43 expression is reduced in the barrel centers and increased in the septa. The present study evaluated the effect of elevated 5-HT levels on the distribution of GAP-43 immunoreactivity in S-I. We employed 5-HT immunocytochemistry and 1,1'-dioctadecyl-3,3,3",3'-tetramethylindocarbocyanine perchlorate (DiI) labeling of thalamic radiations to confirm a 'barrelless' phenotype in P-6 clorgyline-treated animals and a recovered barrel pattern in treated animals allowed to survive until P-8 and P-10. GAP-43 immunocytochemistry was used to evaluate the cortical distribution of this protein in similarly treated littermates. Continuous inhibition of MAO(A) from P-0 to P-6 resulted in a corresponding loss of the GAP-43 vibrissae-related pattern at P-6. Despite continued elevation of cortical 5-HT until P-8 and P-10, the characteristic vibrissae-complementary pattern of GAP-43 emerged with expression concentrated in the septa and rows. GAP-43 vibrissae-related thalamocortical axon pattern never appeared in the clorgyline-treated animals. Thus, while elevated 5-HT delays development of a vibrissae-related pattern of thalamocortical afferents, it does not appear to alter the time when a GAP-43 vibrissae-related complementary pattern emerges.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0165-3806
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
139
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
167-74
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed-meshheading:12480131-Animals, pubmed-meshheading:12480131-Animals, Newborn, pubmed-meshheading:12480131-Carbocyanines, pubmed-meshheading:12480131-Cell Differentiation, pubmed-meshheading:12480131-Clorgyline, pubmed-meshheading:12480131-Female, pubmed-meshheading:12480131-GAP-43 Protein, pubmed-meshheading:12480131-Gene Expression Regulation, Developmental, pubmed-meshheading:12480131-Immunohistochemistry, pubmed-meshheading:12480131-Male, pubmed-meshheading:12480131-Monoamine Oxidase Inhibitors, pubmed-meshheading:12480131-Neural Pathways, pubmed-meshheading:12480131-Neurons, pubmed-meshheading:12480131-Rats, pubmed-meshheading:12480131-Rats, Sprague-Dawley, pubmed-meshheading:12480131-Serotonin, pubmed-meshheading:12480131-Somatosensory Cortex, pubmed-meshheading:12480131-Thalamus, pubmed-meshheading:12480131-Up-Regulation, pubmed-meshheading:12480131-Vibrissae
pubmed:year
2002
pubmed:articleTitle
Effects of elevated serotonin levels on patterns of GAP-43 expression during barrel development in rat somatosensory cortex.
pubmed:affiliation
Department of Anatomy and Neurobiology, Medical College of Ohio, 3000 Arlington Avenue, Toledo, OH 43614, USA. kkesterson@mco.edu
pubmed:publicationType
Journal Article