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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
26
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pubmed:dateCreated |
2002-12-24
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pubmed:abstractText |
With an ever-increasing resource of validated single-nucleotide polymorphisms (SNPs), the limiting factors in genome-wide association analysis have become genotyping capacity and the availability of DNA. We provide a proof of concept of the use of pooled DNA as a means of efficiently screening SNPs and prioritizing them for further study. This approach reduces the final number of SNPs that undergo full, sample-by-sample genotyping as well as the quantity of DNA used overall. We have examined 15 SNPs in the cholesteryl ester transfer protein (CETP) gene, a gene previously demonstrated to be associated with serum high-density lipoprotein cholesterol levels. The SNPs were amplified in two pools of DNA derived from groups of individuals with extremely high and extremely low serum high-density lipoprotein cholesterol levels, respectively. P values <0.05 were obtained for 14 SNPs, supporting the described association. Genotyping of the individual samples showed that the average margin of error in frequency estimate was approximately 4% when pools were used. These findings clearly demonstrate the potential of pooling techniques and their associated technologies as an initial screen in the search for genetic associations.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/12475937-10369254,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12475937-10611357,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12475937-10631153,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12475937-10669650,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12475937-10788337,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12475937-10861897,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12475937-11083947,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12475937-11136232,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12475937-11346797,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12475937-1980239,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12475937-2184120,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12475937-2564326,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12475937-3600759,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12475937-3867663,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12475937-3937535,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12475937-7887436,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12475937-8801636,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12475937-9102177,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12475937-9326338,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12475937-9477339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12475937-9683608,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12475937-9872982
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
24
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pubmed:volume |
99
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
16871-4
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:12475937-Adolescent,
pubmed-meshheading:12475937-Adult,
pubmed-meshheading:12475937-Aged,
pubmed-meshheading:12475937-Alleles,
pubmed-meshheading:12475937-Carrier Proteins,
pubmed-meshheading:12475937-Cholesterol, HDL,
pubmed-meshheading:12475937-Cholesterol Ester Transfer Proteins,
pubmed-meshheading:12475937-Female,
pubmed-meshheading:12475937-Gene Pool,
pubmed-meshheading:12475937-Glycoproteins,
pubmed-meshheading:12475937-Haplotypes,
pubmed-meshheading:12475937-Humans,
pubmed-meshheading:12475937-Middle Aged,
pubmed-meshheading:12475937-Polymorphism, Single Nucleotide
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pubmed:year |
2002
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pubmed:articleTitle |
Association testing by DNA pooling: an effective initial screen.
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pubmed:affiliation |
SEQUENOM-Gemini Ltd., 162 Cambridge Science Park, Milton Road, Cambridge, CB4 0GH England.
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pubmed:publicationType |
Journal Article
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