| pubmed-article:12475645 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12475645 | lifeskim:mentions | umls-concept:C0025936 | lld:lifeskim |
| pubmed-article:12475645 | lifeskim:mentions | umls-concept:C0059377 | lld:lifeskim |
| pubmed-article:12475645 | lifeskim:mentions | umls-concept:C0011155 | lld:lifeskim |
| pubmed-article:12475645 | lifeskim:mentions | umls-concept:C0152058 | lld:lifeskim |
| pubmed-article:12475645 | lifeskim:mentions | umls-concept:C0332120 | lld:lifeskim |
| pubmed-article:12475645 | pubmed:issue | 7 | lld:pubmed |
| pubmed-article:12475645 | pubmed:dateCreated | 2002-12-11 | lld:pubmed |
| pubmed-article:12475645 | pubmed:abstractText | Previous studies have shown that inhibition of nidogen-laminin binding interferes with basement membrane stabilization in various mouse organ cultures while no overt phenotype has been observed following inactivation of the nidogen-1 gene in mice. We have now used recombinant mouse nidogen-1 and nidogen-2 in order to evaluate a possible compensation between the two isoforms in the knock-out mice. Essentially, a comparable in vitro binding of nidogens-1 and -2 to the same laminin gamma1 chain structure and to several other basement membrane proteins has been revealed. Quantitative radioimmuno-assays have demonstrated high concentrations of nidogen-1 exceeding those of laminin gamma1 and nidogen-2 by factors of 5 and 20-50, respectively, in tissue extracts of wild-type mice. A three- to sevenfold increase in nidogen-2 was observed in heart and muscle of mice with nidogen-1 deficiency and confirmed by a similar increase in the intensity of immunogold staining of these tissues. However, a few of the tissues from mice with the gene knock-out still contained some nidogen-1-like immunoreactivity (1% of wild-type). Furthermore, both nidogen isoforms showed a similar distribution in various organs during embryonic development which, however, as shown previously, changed in some adult tissues. The data support the nidogen-2 compensation hypothesis to explain the limited phenotype observed following elimination of the nidogen-1 gene. | lld:pubmed |
| pubmed-article:12475645 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12475645 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12475645 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:12475645 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12475645 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:12475645 | pubmed:issn | 0945-053X | lld:pubmed |
| pubmed-article:12475645 | pubmed:author | pubmed-author:TimplRupertR | lld:pubmed |
| pubmed-article:12475645 | pubmed:author | pubmed-author:SasakiTakakoT | lld:pubmed |
| pubmed-article:12475645 | pubmed:author | pubmed-author:MiosgeNicolai... | lld:pubmed |
| pubmed-article:12475645 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12475645 | pubmed:volume | 21 | lld:pubmed |
| pubmed-article:12475645 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12475645 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12475645 | pubmed:pagination | 611-21 | lld:pubmed |
| pubmed-article:12475645 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
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| pubmed-article:12475645 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:12475645 | pubmed:articleTitle | Evidence of nidogen-2 compensation for nidogen-1 deficiency in transgenic mice. | lld:pubmed |
| pubmed-article:12475645 | pubmed:affiliation | Department of Histology, Georg-August-Universität Göttingen, D-37075, Göttingen, Germany. nmiosge@gwdg.de | lld:pubmed |
| pubmed-article:12475645 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12475645 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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