Source:http://linkedlifedata.com/resource/pubmed/id/12475645
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
7
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pubmed:dateCreated |
2002-12-11
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pubmed:abstractText |
Previous studies have shown that inhibition of nidogen-laminin binding interferes with basement membrane stabilization in various mouse organ cultures while no overt phenotype has been observed following inactivation of the nidogen-1 gene in mice. We have now used recombinant mouse nidogen-1 and nidogen-2 in order to evaluate a possible compensation between the two isoforms in the knock-out mice. Essentially, a comparable in vitro binding of nidogens-1 and -2 to the same laminin gamma1 chain structure and to several other basement membrane proteins has been revealed. Quantitative radioimmuno-assays have demonstrated high concentrations of nidogen-1 exceeding those of laminin gamma1 and nidogen-2 by factors of 5 and 20-50, respectively, in tissue extracts of wild-type mice. A three- to sevenfold increase in nidogen-2 was observed in heart and muscle of mice with nidogen-1 deficiency and confirmed by a similar increase in the intensity of immunogold staining of these tissues. However, a few of the tissues from mice with the gene knock-out still contained some nidogen-1-like immunoreactivity (1% of wild-type). Furthermore, both nidogen isoforms showed a similar distribution in various organs during embryonic development which, however, as shown previously, changed in some adult tissues. The data support the nidogen-2 compensation hypothesis to explain the limited phenotype observed following elimination of the nidogen-1 gene.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Adhesion Molecules,
http://linkedlifedata.com/resource/pubmed/chemical/Laminin,
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/NID2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/laminin gamma 1,
http://linkedlifedata.com/resource/pubmed/chemical/nidogen
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0945-053X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
21
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
611-21
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:12475645-Adaptation, Physiological,
pubmed-meshheading:12475645-Animals,
pubmed-meshheading:12475645-Carrier Proteins,
pubmed-meshheading:12475645-Cell Adhesion Molecules,
pubmed-meshheading:12475645-Embryo, Mammalian,
pubmed-meshheading:12475645-Embryonic and Fetal Development,
pubmed-meshheading:12475645-Immunologic Techniques,
pubmed-meshheading:12475645-Laminin,
pubmed-meshheading:12475645-Ligands,
pubmed-meshheading:12475645-Membrane Glycoproteins,
pubmed-meshheading:12475645-Mice,
pubmed-meshheading:12475645-Mice, Inbred Strains,
pubmed-meshheading:12475645-Mice, Knockout,
pubmed-meshheading:12475645-Phenotype,
pubmed-meshheading:12475645-Recombinant Proteins,
pubmed-meshheading:12475645-Up-Regulation
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pubmed:year |
2002
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pubmed:articleTitle |
Evidence of nidogen-2 compensation for nidogen-1 deficiency in transgenic mice.
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pubmed:affiliation |
Department of Histology, Georg-August-Universität Göttingen, D-37075, Göttingen, Germany. nmiosge@gwdg.de
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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