Linked Life Data

12474542

Source:http://linkedlifedata.com/resource/pubmed/id/12474542

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2002-12-11
pubmed:abstractText
The molecular basis for the loss of KAI1 expression in invasive and metastatic tumors and tumor cell lines is not understood. Recently, identification of a sequence with homology to the consensus p53-binding motif in the promoter of the KAI1 metastasis suppressor gene, has led to a proposal that transcriptional regulation by p53 controls expression of KAI1, and that a dramatic down-regulation of KAI1 mRNA levels in invasive tumors and many tumor cell lines, is directly due to loss of p53 function. We have tested this hypothesis by assessing KAI1 mRNA levels in a series of 22 cell lines derived from bladder and prostate cancers, in which we confirmed the p53 gene sequence and characterized the functional status of the endogenous p53 protein. We anticipated that cell lines expressing p53 capable of transactivation should express high levels of KAI1 mRNA compared with cell lines expressing defective p53, or which were p53-null. KAI1 mRNA levels were determined by northern analysis using a full-length KAI1 cDNA probe, and varied widely between cell lines examined. However, there was no association between these levels and p53 status. Furthermore, transfection of representative cell lines with wild-type p53, or exposure to DNA damaging agents, had no effect on KAI1 mRNA levels. Our data suggest that p53 is not a major factor regulating levels of KAI1 mRNA in bladder and prostate cancer cell lines.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1078-1439
pubmed:author
pubmed:issnType
Print
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
99-104
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:12474542-Antigens, CD, pubmed-meshheading:12474542-Antigens, CD82, pubmed-meshheading:12474542-Base Sequence, pubmed-meshheading:12474542-DNA Primers, pubmed-meshheading:12474542-Genes, Tumor Suppressor, pubmed-meshheading:12474542-Humans, pubmed-meshheading:12474542-Male, pubmed-meshheading:12474542-Membrane Glycoproteins, pubmed-meshheading:12474542-Promoter Regions, Genetic, pubmed-meshheading:12474542-Prostatic Neoplasms, pubmed-meshheading:12474542-Proto-Oncogene Proteins, pubmed-meshheading:12474542-RNA, Messenger, pubmed-meshheading:12474542-Recombinant Proteins, pubmed-meshheading:12474542-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:12474542-Transcription, Genetic, pubmed-meshheading:12474542-Transfection, pubmed-meshheading:12474542-Tumor Cells, Cultured, pubmed-meshheading:12474542-Tumor Suppressor Protein p53, pubmed-meshheading:12474542-Urinary Bladder Neoplasms
pubmed:articleTitle
Relationship between expression of KAI1 metastasis suppressor gene, mRNA levels and p53 in human bladder and prostate cancer cell lines.
pubmed:affiliation
Oncology Research Centre, Prince of Wales Hospital, School of Medicine, University of New South Wales, Barker Street, Randwick, NSW 2031, Australia. paul.jackson@unsw.edu.au
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't