Linked Life Data

12472893

Source:http://linkedlifedata.com/resource/pubmed/id/12472893

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2002-12-10
pubmed:abstractText
In this study we investigated the mRNA expression of NELL2, a neural tissue-specific epidermal growth factor (EGF)-like repeat domain-containing protein, in the developing and adult rat CNS using in situ hybridization histochemistry and northern blot analysis. The possible candidates that interact with or be regulated by NELL2 were screened with a cDNA expression array in antisense (AS) NELL2 oligodeoxynucleotide (ODN)-injected rat hypothalami. NELL2 mRNA was detected as early as embryonic day 10, and was predominant in the CNS throughout the pre-natal stages. Its expression gradually increased during embryonic development and its strong expression was observed throughout the CNS until embryonic day 20. It was detected in the ventricular zone of the spinal cord, medulla and pons in 12-day-old-embryos, suggesting that NELL2 plays a role in the neurogenesis of these areas. After birth its expression gradually decreased, but high levels of expression could be observed in the tenia tecta, piriform cortex, hippocampus, dentate gyrus, cerebellar cortex, ambiguus nucleus, and inferior olivary nucleus of adult rat brains. The analysis of cDNA expression arrays revealed that the administration of AS NELL2 ODN markedly decreased the expression of several Ca2+-binding proteins and those involved in the transport and release of vesicles such as EF-hand Ca2+-binding protein p22 and rab7. This finding was confirmed by relative reverse transcription-polymerase chain reaction. The effect of NELL2 on synaptic vesicle content in median eminence (ME) nerve terminals was determined with synaptophysin levels as a marker protein in the AS NELL2 ODN-injected rat. It was significantly decreased by the AS ODN. These data suggest that NELL2 may play an important role in the development of the CNS as well as maintenance of neural functions, by regulating the intracellular machinery involving Ca2+ signaling, synaptic transport and/or release of vesicles.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0022-3042
pubmed:author
pubmed:issnType
Print
pubmed:volume
83
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1389-400
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12472893-Animals, pubmed-meshheading:12472893-Blotting, Northern, pubmed-meshheading:12472893-Brain, pubmed-meshheading:12472893-Epidermal Growth Factor, pubmed-meshheading:12472893-Hypothalamus, pubmed-meshheading:12472893-Immunohistochemistry, pubmed-meshheading:12472893-In Situ Hybridization, pubmed-meshheading:12472893-Injections, Intraventricular, pubmed-meshheading:12472893-Nerve Tissue Proteins, pubmed-meshheading:12472893-Neurons, pubmed-meshheading:12472893-Oligonucleotide Array Sequence Analysis, pubmed-meshheading:12472893-Oligonucleotides, Antisense, pubmed-meshheading:12472893-Organ Specificity, pubmed-meshheading:12472893-Protein Structure, Tertiary, pubmed-meshheading:12472893-RNA, Messenger, pubmed-meshheading:12472893-Rats, pubmed-meshheading:12472893-Rats, Sprague-Dawley, pubmed-meshheading:12472893-Repetitive Sequences, Amino Acid, pubmed-meshheading:12472893-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:12472893-Spinal Cord, pubmed-meshheading:12472893-Synaptic Vesicles, pubmed-meshheading:12472893-Synaptophysin
pubmed:year
2002
pubmed:articleTitle
Ontogeny and the possible function of a novel epidermal growth factor-like repeat domain-containing protein, NELL2, in the rat brain.
pubmed:affiliation
Department of Anatomy, Brain Korea 21 Biomedical Sciences, Korea University College of Medicine, Seoul, South Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't