|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
Pt 1
|
|
pubmed:dateCreated |
2003-3-20
|
|
pubmed:abstractText |
The tendency for tetramerization is the main disadvantage in the green fluorescent protein homologues from Anthozoa species. We report a universal method called hetero-oligomeric tagging, which diminishes troublesome consequences of tetramerization of Anthozoa-derived fluorescent proteins (FP) in intracellular protein labelling. This approach is based on the co-expression of the FP-tagged protein of interest together with an excess of free non-fluorescent FP mutant. The resulting FP heterotetramers contain only a single target polypeptide and, therefore, can be considered pseudo-monomeric. Feasibility of the method has been demonstrated with a red FP fused with cytoplasmic beta-actin or tubulin-binding protein Tau34. In addition, heterotetramers appeared to be a unique model for biophysical characterization of Anthozoa FPs in pseudo-monomeric state.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/12472468-10504696,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12472468-10852900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12472468-10929761,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12472468-10981720,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12472468-11050229,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12472468-11050231,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12472468-11150510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12472468-11327872,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12472468-11389889,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12472468-11682051,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12472468-11724943,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12472468-11724946,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12472468-11773062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12472468-11812239,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12472468-11821040,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12472468-12060735,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12472468-12271129,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12472468-12350221,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12472468-12419311,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12472468-12524551,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12472468-2744487,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12472468-8707048,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12472468-9759496
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Apr
|
|
pubmed:issn |
0264-6021
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
1
|
|
pubmed:volume |
371
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
109-14
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:12472468-Actins,
pubmed-meshheading:12472468-Animals,
pubmed-meshheading:12472468-Anthozoa,
pubmed-meshheading:12472468-Biochemistry,
pubmed-meshheading:12472468-CHO Cells,
pubmed-meshheading:12472468-Cricetinae,
pubmed-meshheading:12472468-Gene Expression Regulation,
pubmed-meshheading:12472468-Genes,
pubmed-meshheading:12472468-Green Fluorescent Proteins,
pubmed-meshheading:12472468-Luminescent Proteins,
pubmed-meshheading:12472468-Microtubule-Associated Proteins,
pubmed-meshheading:12472468-Mutation,
pubmed-meshheading:12472468-Protein Binding,
pubmed-meshheading:12472468-Protein Conformation,
pubmed-meshheading:12472468-Protein Engineering,
pubmed-meshheading:12472468-Recombinant Fusion Proteins
|
|
pubmed:year |
2003
|
|
pubmed:articleTitle |
Hetero-oligomeric tagging diminishes non-specific aggregation of target proteins fused with Anthozoa fluorescent proteins.
|
|
pubmed:affiliation |
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry RAS, Miklukho-Maklaya 16/10, Moscow 117997, Russia.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
