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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2002-12-9
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pubmed:abstractText |
T-614 (N-[3-(formylamino)-4-oxo-6-phenoxy-4H-chromen-7-yl]methanesulfonamide), a newly developed anti-rheumatic drug under clinical trial, is an anti-inflammatory agent which has been reported to show the inhibitory effect of bone destruction in vivo arthritis model. We found that T-614 stimulated osteoblastic differentiation of stromal cell line (ST2) and preosteoblastic cell line (MC3T3-E1) in the presence or absence of recombinant human bone morphogenetic protein-2 (rhBMP-2). Calcium content of mineralized nodules was 14-fold elevated by the addition of T-614 in the presence of rhBMP-2 in ST2 but not MC3T3-E1. Oral administration of T-614 to mice also promoted rhBMP-2 induced bone formation in vivo. Northern blot analysis showed that transcriptional level of osterix, an essential transcription factor for osteoblastic differentiation, was 3-fold increased by T-614 with rhBMP-2 in ST2. Taken together, these results suggested that T-614 possessed anabolic effects on bone metabolism, besides suppressor of bone resorption, by increased expression of osterix.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Alkaline Phosphatase,
http://linkedlifedata.com/resource/pubmed/chemical/Antirheumatic Agents,
http://linkedlifedata.com/resource/pubmed/chemical/BMP2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Benzopyrans,
http://linkedlifedata.com/resource/pubmed/chemical/Bmp2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Bone Morphogenetic Protein 2,
http://linkedlifedata.com/resource/pubmed/chemical/Bone Morphogenetic Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Osteocalcin,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Sulfonamides,
http://linkedlifedata.com/resource/pubmed/chemical/T 614,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
20
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pubmed:volume |
299
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
903-9
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:12470665-Alkaline Phosphatase,
pubmed-meshheading:12470665-Animals,
pubmed-meshheading:12470665-Antirheumatic Agents,
pubmed-meshheading:12470665-Benzopyrans,
pubmed-meshheading:12470665-Bone Morphogenetic Protein 2,
pubmed-meshheading:12470665-Bone Morphogenetic Proteins,
pubmed-meshheading:12470665-Bone and Bones,
pubmed-meshheading:12470665-Cell Differentiation,
pubmed-meshheading:12470665-Cell Line,
pubmed-meshheading:12470665-Dose-Response Relationship, Drug,
pubmed-meshheading:12470665-Drug Synergism,
pubmed-meshheading:12470665-Mice,
pubmed-meshheading:12470665-Neoplasm Proteins,
pubmed-meshheading:12470665-Osteoblasts,
pubmed-meshheading:12470665-Osteocalcin,
pubmed-meshheading:12470665-Osteogenesis,
pubmed-meshheading:12470665-RNA, Messenger,
pubmed-meshheading:12470665-Stromal Cells,
pubmed-meshheading:12470665-Sulfonamides,
pubmed-meshheading:12470665-Transcription Factors,
pubmed-meshheading:12470665-Transforming Growth Factor beta
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pubmed:year |
2002
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pubmed:articleTitle |
A novel anti-rheumatic drug, T-614, stimulates osteoblastic differentiation in vitro and bone morphogenetic protein-2-induced bone formation in vivo.
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pubmed:affiliation |
Department of Biology, Osaka Medical Center for Cancer and Cardiovascular Diseases, 1-3-2 Nakamichi, Higashinari-ku, Osaka 537-8511, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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