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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2002-12-9
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pubmed:abstractText |
Quantifying cytokines on the protein level can be problematic because of low concentrations or degradation during sample handling. Aiming towards finding a simple method by which to quantify cytokines on the mRNA level, we combined existing and established molecular biology techniques. Based on the principle of quantitative competitive RT-PCR with a DNA-competitor, IL-1beta, IL-6, IL-12alpha and the housekeeping enzyme GAPDH are measured at levels down to 200 copies of mRNA.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0036-5513
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
62
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
405-12
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12469895-Cell Line,
pubmed-meshheading:12469895-Cytokines,
pubmed-meshheading:12469895-DNA, Complementary,
pubmed-meshheading:12469895-Interleukin-1,
pubmed-meshheading:12469895-Interleukin-12,
pubmed-meshheading:12469895-Interleukin-6,
pubmed-meshheading:12469895-Lipopolysaccharides,
pubmed-meshheading:12469895-Monocytes,
pubmed-meshheading:12469895-Polymerase Chain Reaction,
pubmed-meshheading:12469895-RNA, Messenger
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pubmed:year |
2002
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pubmed:articleTitle |
LPS-induced cytokine production in the monocytic cell line THP-1 determined by multiple quantitative competitive PCR (QC-PCR).
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pubmed:affiliation |
Laboratory of Medical Allergology, Allergy Unit, National University Hospital, Copenhagen, Denmark. cglue@rh.dk
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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