Source:http://linkedlifedata.com/resource/pubmed/id/12466473
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
Pt 12
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pubmed:dateCreated |
2002-12-5
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pubmed:abstractText |
The potential of CpG-enhanced plasmid DNA vectors encoding a truncated secreted form of bovine herpesvirus-1 (BHV-1) glycoprotein D (tgD) to induce enhanced immune responses in cattle was investigated. We created tgD expression plasmids containing 0, 40 or 88 copies of the hexamer 5' GTCGTT 3', a known pan-activating CpG motif in several species. The total tgD-specific IgG titre of calves immunized with these plasmids did not correlate with the CpG content of the plasmid backbone. However, the pBISIA88-tgD-vaccinated group showed a significantly lower IgG1:IgG2 ratio than calves immunized with pBISIA40-tgD or pMASIA-tgD, which has no CpG motifs inserted. Antigen-specific lymphocyte proliferation and IFN-gamma secretion by peripheral blood mononuclear cells correlated positively with the CpG content of the vectors. In contrast, calves that received a killed BHV-1 vaccine had an IgG1-predominant isotype and low lymphocyte proliferation and IFN-gamma levels. Following challenge, the pBISIA88-tgD-immunized group developed the greatest anamnestic response, the highest BHV-1 neutralization titres in serum and a significantly lower level of virus shedding than the saline control group. However, there were no significant differences in clinical symptoms of infection between the DNA-immunized groups and the saline control group. These data indicate that CpG-enhanced plasmids induce augmented immune responses and could be used to vaccinate against pathogens requiring a strong cellular response for protection.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Herpesvirus Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/bovine herpesvirus type-1...
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0022-1317
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
83
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2973-81
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:12466473-Animals,
pubmed-meshheading:12466473-Antibodies, Viral,
pubmed-meshheading:12466473-Base Sequence,
pubmed-meshheading:12466473-Cattle,
pubmed-meshheading:12466473-Cattle Diseases,
pubmed-meshheading:12466473-CpG Islands,
pubmed-meshheading:12466473-Genetic Vectors,
pubmed-meshheading:12466473-Herpesviridae Infections,
pubmed-meshheading:12466473-Herpesvirus 1, Bovine,
pubmed-meshheading:12466473-Herpesvirus Vaccines,
pubmed-meshheading:12466473-Immunization,
pubmed-meshheading:12466473-Interferon-gamma,
pubmed-meshheading:12466473-Lymphocyte Activation,
pubmed-meshheading:12466473-Plasmids,
pubmed-meshheading:12466473-Vaccines, DNA,
pubmed-meshheading:12466473-Viral Proteins
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pubmed:year |
2002
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pubmed:articleTitle |
Augmentation of cellular immune responses to bovine herpesvirus-1 glycoprotein D by vaccination with CpG-enhanced plasmid vectors.
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pubmed:affiliation |
Veterinary Infectious Disease Organization, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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