Source:http://linkedlifedata.com/resource/pubmed/id/12466273
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
8
|
| pubmed:dateCreated |
2003-2-17
|
| pubmed:abstractText |
Accumulation of low-density lipoprotein (LDL)-derived cholesterol by macrophages in vessel walls is a pathogenomic feature of atherosclerotic lesions. Platelets contribute to lipid uptake by macrophages through mechanisms that are only partially understood. We have previously shown that platelet factor 4 (PF4) inhibits the binding and degradation of LDL through its receptor, a process that could promote the formation of oxidized LDL (ox-LDL). We have now characterized the effect of PF4 on the binding of ox-LDL to vascular cells and macrophages and on the accumulation of cholesterol esters. PF4 bound to ox-LDL directly and also increased ox-LDL binding to vascular cells and macrophages. PF4 did not stimulate ox-LDL binding to cells that do not synthesize glycosaminoglycans or after enzymatic cleavage of cell surface heparan and chondroitin sulfates. The effect of PF4 on binding ox-LDL was dependent on specific lysine residues in its C terminus. Addition of PF4 also caused an approximately 10-fold increase in the amount of ox-LDL esterified by macrophages. Furthermore, PF4 and ox-LDL co-localize in atherosclerotic lesion, especially in macrophage-derived foam cells. These observations offer a potential mechanism by which platelet activation at sites of vascular injury may promote the accumulation of deleterious lipoproteins and offer a new focus for pharmacological intervention in the development of atherosclerosis.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Lipoproteins, LDL,
http://linkedlifedata.com/resource/pubmed/chemical/Platelet Factor 4,
http://linkedlifedata.com/resource/pubmed/chemical/Proteoglycans,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/oxidized low density lipoprotein
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0021-9258
|
| pubmed:author |
pubmed-author:AkkawiSa'edS,
pubmed-author:AviramMichaelM,
pubmed-author:BdeirKhalilK,
pubmed-author:CinesDouglasD,
pubmed-author:HigaziAbd Al-RoofAA,
pubmed-author:HissEdnaE,
pubmed-author:KowalskaMaria AnnaMA,
pubmed-author:LeitersdorfEranE,
pubmed-author:NassarTaherT,
pubmed-author:PonczMortimerM,
pubmed-author:SachaisBruce SBS,
pubmed-author:ZiporenLeahL
|
| pubmed:issnType |
Print
|
| pubmed:day |
21
|
| pubmed:volume |
278
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
6187-93
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:12466273-Amino Acid Substitution,
pubmed-meshheading:12466273-Animals,
pubmed-meshheading:12466273-Arteriosclerosis,
pubmed-meshheading:12466273-Blood Platelets,
pubmed-meshheading:12466273-CHO Cells,
pubmed-meshheading:12466273-Cells, Cultured,
pubmed-meshheading:12466273-Cricetinae,
pubmed-meshheading:12466273-Endothelium, Vascular,
pubmed-meshheading:12466273-Genetic Variation,
pubmed-meshheading:12466273-Humans,
pubmed-meshheading:12466273-Immunohistochemistry,
pubmed-meshheading:12466273-Lipoproteins, LDL,
pubmed-meshheading:12466273-Microscopy, Confocal,
pubmed-meshheading:12466273-Mutagenesis, Site-Directed,
pubmed-meshheading:12466273-Platelet Factor 4,
pubmed-meshheading:12466273-Protein Binding,
pubmed-meshheading:12466273-Proteoglycans,
pubmed-meshheading:12466273-Recombinant Proteins,
pubmed-meshheading:12466273-Transfection,
pubmed-meshheading:12466273-Umbilical Veins
|
| pubmed:year |
2003
|
| pubmed:articleTitle |
Platelet factor 4 enhances the binding of oxidized low-density lipoprotein to vascular wall cells.
|
| pubmed:affiliation |
Department of Clinical Biochemistry, the Center for Research, Prevention, and Treatment of Atherosclerosis, Hadassah University Hospital and Hebrew University-Hadassah Medical School, Jerusalem 91120, Israel.
|
| pubmed:publicationType |
Journal Article
|