|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
8
|
|
pubmed:dateCreated |
2002-12-5
|
|
pubmed:abstractText |
1. The aim of this study was to characterize the 5-HT receptors involved in the 5-HT-induced contraction of longitudinal muscle (LM) strips of porcine proximal stomach. This was done in a classical organ bath set-up for isotonic measurement. 2. The concentration-contraction curve to 5-HT was not modified by 5-HT(3) and 5-HT(4) receptor antagonism. Methysergide, ketanserin and mesulergine antagonized the curve to 5-HT. Concomitantly, increasing concentrations of ketanserin and mesulergine progressively revealed a biphasic nature of the 5-HT curve. Ketanserin antagonized the low-affinity receptor while it did not modify the high-affinity receptor. 3. Tetrodotoxin did not influence the concentration-contraction curve to 5-HT neither in the absence nor presence of ketanserin, indicating that nerves are not involved. 4. Ketanserin competitively antagonized the monophasic concentration-response curve to alpha-Methyl-5-HT, yielding a Schild slope that was not significantly different from unity. After constraining the Schild slope to unity, a pK(B) estimate of 8.23+/-0.90 was obtained. This affinity estimate of ketanserin closely approximates previously reported affinities at 5-HT(2A) receptors. 5. In the presence of ketanserin (0.1 microM; exposing the high-affinity receptor), a wide range of 5-HT receptor antagonists covering all 5-HT receptors known, was tested. Only methysergide and ritanserin inhibited the response to 5-HT, thus expressing affinity for the high-affinity receptor. This did not reveal the identity of the receptor involved. 6 It can be concluded that 5-HT induces pig proximal stomach (LM) contraction via 5-HT(2A) receptors located on smooth muscle. A ketanserin-insensitive phase of contractions could not be characterized between the actually known classes of 5-HT receptors with the pharmacological tools that were used.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/12466231-10401550,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12466231-10716674,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12466231-11309267,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12466231-11704657,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12466231-12010782,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12466231-1451742,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12466231-1886080,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12466231-2540014,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12466231-2866002,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12466231-3288820,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12466231-3300739,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12466231-3416910,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12466231-7506394,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12466231-7699699,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12466231-7813582,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12466231-7938165,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12466231-8012715,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12466231-8032658,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12466231-8741174,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12466231-8903510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12466231-9051313,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12466231-9200559,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12466231-9298538,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12466231-9484865,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12466231-9950810
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Dec
|
|
pubmed:issn |
0007-1188
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
137
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1217-24
|
|
pubmed:dateRevised |
2010-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:12466231-Animals,
pubmed-meshheading:12466231-Dose-Response Relationship, Drug,
pubmed-meshheading:12466231-Female,
pubmed-meshheading:12466231-Male,
pubmed-meshheading:12466231-Muscle, Smooth,
pubmed-meshheading:12466231-Muscle Contraction,
pubmed-meshheading:12466231-Receptor, Serotonin, 5-HT2A,
pubmed-meshheading:12466231-Receptors, Serotonin,
pubmed-meshheading:12466231-Serotonin Antagonists,
pubmed-meshheading:12466231-Serotonin Receptor Agonists,
pubmed-meshheading:12466231-Stomach,
pubmed-meshheading:12466231-Swine
|
|
pubmed:year |
2002
|
|
pubmed:articleTitle |
Smooth muscle 5-HT2A receptors mediating contraction of porcine isolated proximal stomach strips.
|
|
pubmed:affiliation |
Heymans Institute of Pharmacology, Ghent University, Ghent, Belgium.
|
|
pubmed:publicationType |
Journal Article,
In Vitro
|
