Linked Life Data

12465409

Source:http://linkedlifedata.com/resource/pubmed/id/12465409

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2002-12-5
pubmed:abstractText
Various methods are available for the assessment of cell viability. Recently, interest has centered on methods using fluorescent dyes. In this work, we used a double-staining assay, involving the use of rhodamin 123, which stains the mitochondria of viable cells, and propidium iodide, which stains the nuclei of dead cells, to investigate their use in assessing the viability of cells. We added adriamycin to NIH 3T3 cells and double-stained the cells that adhered to the dish and those that were suspended in the culture solution, and observed the results over time. We found that nearly all the adherent cells were stained with rhodamin 123 alone. However, the suspended cells in the control group accounted for most of the double-stained cells, and when adriamycin was added, most were stained with PI alone.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0385-0684
pubmed:author
pubmed:issnType
Print
pubmed:volume
29
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2025-9
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Evaluation of cell viability by double-staining fluorescence assay.
pubmed:affiliation
Department of Surgery II, Nippon Medical School, 1-1-5, Sendagi, Bunkyo-ku, Tokyo, 113-8603, Japan.
pubmed:publicationType
Journal Article