Linked Life Data

12461751

Source:http://linkedlifedata.com/resource/pubmed/id/12461751

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2002-12-3
pubmed:abstractText
The tumor-suppressor gene PTEN (phosphatase and tensin homolog) is frequently inactivated in different types of human tumors. Less is known about the involvement of the homologous gene Pten in animal model systems of cancer. By sequencing one of the introns of rat Pten, we found an informative intragenic PCR marker suitable for genetic studies. Through use of this marker, the position of Pten in the genetic linkage map was localized to the distal part of rat chromosome 1 (RNO1) by analysis of F2 progeny from an intercross between inbred strains BN and LE. Subsequently, 22 markers from this region (including the intragenic Pten marker) were used to study the occurrence of allelic imbalance in distal RNO1 in fibrosarcomas that had been induced by DMBA in F1(BNxLE) rats. The analysis revealed that allelic imbalance was common in the vicinity of Pten, and there was loss or reduction of one of the Pten alleles in more than 60% of the fibrosarcomas. DNA sequencing was preformed to investigate whether the Pten allele remaining in the tumors was inactivated by mutation. However, no mutations were detected in the genomic sequence of Pten exons 5 to 9 in any of the fibrosarcomas, and normal mRNA transcripts were expressed in all tumors. Thus, based on the targeted selection for loss of Pten observed in some of these tumors and the absence of inactivation of the remaining allele, we suggest that haploinsufficiency of Pten may be an important factor in rat DMBA-induced fibrosarcomas.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1045-2257
pubmed:author
pubmed:copyrightInfo
Copyright 2002 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:volume
36
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
70-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12461751-9,10-Dimethyl-1,2-benzanthracene, pubmed-meshheading:12461751-Allelic Imbalance, pubmed-meshheading:12461751-Animals, pubmed-meshheading:12461751-Carcinogens, pubmed-meshheading:12461751-Chromosome Mapping, pubmed-meshheading:12461751-Chromosomes, pubmed-meshheading:12461751-Fibrosarcoma, pubmed-meshheading:12461751-Genes, Tumor Suppressor, pubmed-meshheading:12461751-Genetic Markers, pubmed-meshheading:12461751-Humans, pubmed-meshheading:12461751-Introns, pubmed-meshheading:12461751-Liver Neoplasms, pubmed-meshheading:12461751-Mice, pubmed-meshheading:12461751-PTEN Phosphohydrolase, pubmed-meshheading:12461751-Phosphoric Monoester Hydrolases, pubmed-meshheading:12461751-Polymorphism, Genetic, pubmed-meshheading:12461751-Rats, pubmed-meshheading:12461751-Rats, Inbred BN, pubmed-meshheading:12461751-Rats, Inbred F344, pubmed-meshheading:12461751-Rats, Inbred Lew, pubmed-meshheading:12461751-Rats, Inbred SHR, pubmed-meshheading:12461751-Rats, Inbred WKY, pubmed-meshheading:12461751-Rats, Sprague-Dawley, pubmed-meshheading:12461751-Tumor Cells, Cultured, pubmed-meshheading:12461751-Tumor Suppressor Proteins
pubmed:year
2003
pubmed:articleTitle
Recurrent allelic imbalance at the rat Pten locus in DMBA-induced fibrosarcomas.
pubmed:affiliation
Department of Cell and Molecular Biology-Genetics, Lundberg Laboratory, Göteborg University, Sweden. Asa.Sjoling@microbio.gu.se
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't