|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
9
|
|
pubmed:dateCreated |
2003-2-24
|
|
pubmed:abstractText |
Bidentate interaction of a T-cell receptor and CD8alphabeta heterodimer with a peptide-MHCI complex is required for the generation of cytotoxic T-lymphocytes. During thymic development, the modification of CD8beta glycans influences major histocompatibility complex class I binding to T-cell precursors called thymocytes. ES mass spectrometry (MS) and tandem MS/MS analysis were used to identify the changes occurring in the CD8beta-glycopeptides during T-cell development. Several threonine residues proximal to the CD8beta Ig headpiece are glycosylated with core-type 1 O-glycans. Non-sialylated glycoforms are present in immature thymocytes but are virtually absent in mature thymocytes. These results suggest how sialylation in a discrete segment of the CD8beta stalk by ST3Gal-1 sialyltransferase creates a molecular developmental switch that affects ligand binding.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Feb
|
|
pubmed:issn |
0021-9258
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
28
|
|
pubmed:volume |
278
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
7240-6
|
|
pubmed:dateRevised |
2007-11-14
|
|
pubmed:meshHeading |
pubmed-meshheading:12459555-Amino Acid Sequence,
pubmed-meshheading:12459555-Animals,
pubmed-meshheading:12459555-Antigens, CD4,
pubmed-meshheading:12459555-Antigens, CD8,
pubmed-meshheading:12459555-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:12459555-Glycopeptides,
pubmed-meshheading:12459555-Glycosylation,
pubmed-meshheading:12459555-Ligands,
pubmed-meshheading:12459555-Major Histocompatibility Complex,
pubmed-meshheading:12459555-Mass Spectrometry,
pubmed-meshheading:12459555-Mice,
pubmed-meshheading:12459555-Mice, Inbred C57BL,
pubmed-meshheading:12459555-Models, Biological,
pubmed-meshheading:12459555-Molecular Sequence Data,
pubmed-meshheading:12459555-N-Acetylneuraminic Acid,
pubmed-meshheading:12459555-Protein Binding,
pubmed-meshheading:12459555-Sialyltransferases,
pubmed-meshheading:12459555-Thymus Gland
|
|
pubmed:year |
2003
|
|
pubmed:articleTitle |
Sialic acid capping of CD8beta core 1-O-glycans controls thymocyte-major histocompatibility complex class I interaction.
|
|
pubmed:affiliation |
Laboratory of Immunobiology, Department of Cancer Immunology & AIDS, Harvard Medical School, Boston, Massachusetts 02115, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
