12453189

Source:http://linkedlifedata.com/resource/pubmed/id/12453189

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0017262, umls-concept:C0043343, umls-concept:C0127093, umls-concept:C0904504, umls-concept:C1171362, umls-concept:C1515670, umls-concept:C1515877, umls-concept:C1879547
pubmed:dateCreated	2002-11-27
pubmed:abstractText	Functional interactions between ligands and their cognate receptors can be investigated using the ability of melanophores from Xenopus laevis to disperse or aggregate their pigment granules in response to alterations in the intracellular levels of second messengers. We have examined the response of long-term lines of cultured melanophores from X. laevis to pituitary adenylate cyclase activating peptide (PACAP), a neuropeptide with vasodilatory activity, and maxadilan, a vasodilatory peptide present in the salivary gland extracts of the blood feeding sand fly. Pituitary adenylate cyclase activating peptide increased the intracellular levels of cyclic adenosine monophosphate (cAMP) and induced pigment dispersion in the pigment cells, confirming that melanophores express an endogenous PACAP receptor. Maxadilan did not induce a response in non-transfected melanophores. When the melanophores were transfected with complementary DNA (cDNA) from the three different members of the PACAP receptor family, maxadilan induced pigment dispersion specifically and cAMP accumulation in melanophores transfected with the cDNA for PAC1 receptors but not VPAC1 or VPAC2 receptors. A melanophore line was generated that stably expresses the PAC1 receptor.
pubmed:grant	http://linkedlifedata.com/resource/pubmed/grant/R01 AR42005
pubmed:language	eng
pubmed:citationSubset	IM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP, http://linkedlifedata.com/resource/pubmed/chemical/Insect Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Neuropeptides, http://linkedlifedata.com/resource/pubmed/chemical/PACAP protein, Xenopus, http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments, http://linkedlifedata.com/resource/pubmed/chemical/Pigments, Biological, http://linkedlifedata.com/resource/pubmed/chemical/Pituitary Adenylate..., http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Pituitary Adenylate..., http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Pituitary Adenylate..., http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Pituitary Hormone, http://linkedlifedata.com/resource/pubmed/chemical/Vasodilator Agents, http://linkedlifedata.com/resource/pubmed/chemical/Xenopus Proteins, http://linkedlifedata.com/resource/pubmed/chemical/maxadilan protein, insect
pubmed:status	MEDLINE
pubmed:author	pubmed-author:BelloYsabelY, pubmed-author:LernerEthanE, pubmed-author:PereiraPhyllisP, pubmed-author:ReddyVermuri BVB, pubmed-author:XXX
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	461-6
pubmed:dateRevised	2007-11-14
pubmed:articleTitle	Maxadilan activates PAC1 receptors expressed in Xenopus laevis xelanophores.
pubmed:affiliation	Department of Dermatology, Cutaneous Biology Research Center, Massachusetts General Hospital/Harvard Medical School, Charlestown, MA 02129, USA.