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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2003-2-3
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pubmed:abstractText |
Wnt growth factors control numerous cell fate decisions in development by altering specific gene expression patterns through the activity of heterodimeric transcriptional activators. These consist of beta-catenin and one of the four members of the T-cell factor (TCF) family of DNA-binding proteins. How can the Wnt/beta-catenin pathway control various sets of target genes in distinct cellular settings with such a limited number of nuclear effectors? Here we asked whether different TCF proteins could perform specific, nonredundant functions at natural beta-catenin/TCF-regulated promoters. We found that TCF4E but not LEF1 supported beta-catenin-dependent activation of the Cdx1 promoter, whereas LEF1 specifically activated the Siamois promoter. Deletion of a C-terminal domain of TCF4E prevented Cdx1 promoter induction. A chimeric protein consisting of LEF1 and the C terminus of TCF4E was fully functional. Therefore, the TCF4E C terminus harbors a promoter-specific transactivation domain. This domain influences the DNA binding properties of TCF4 and additionally mediates an interaction with the transcriptional coactivator p300. Apparently, the C terminus of TCF4E cooperates with beta-catenin and p300 to form a specialized transcription factor complex that specifically supports the activation of the Cdx1 promoter.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CDX1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/CTNNB1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Catnb protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Cdx1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Cytoskeletal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/E1A-Associated p300 Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Ep300 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Homeodomain Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/TCF Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/TCF7L2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Tcf7l2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor 7-Like 2...,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/beta Catenin
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
7
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pubmed:volume |
278
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3776-85
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:12446687-Animals,
pubmed-meshheading:12446687-Base Sequence,
pubmed-meshheading:12446687-Cytoskeletal Proteins,
pubmed-meshheading:12446687-E1A-Associated p300 Protein,
pubmed-meshheading:12446687-Electrophoretic Mobility Shift Assay,
pubmed-meshheading:12446687-Enhancer Elements, Genetic,
pubmed-meshheading:12446687-Homeodomain Proteins,
pubmed-meshheading:12446687-Humans,
pubmed-meshheading:12446687-Mice,
pubmed-meshheading:12446687-Nuclear Proteins,
pubmed-meshheading:12446687-Phosphorylation,
pubmed-meshheading:12446687-Promoter Regions, Genetic,
pubmed-meshheading:12446687-TCF Transcription Factors,
pubmed-meshheading:12446687-Trans-Activators,
pubmed-meshheading:12446687-Transcription Factor 7-Like 2 Protein,
pubmed-meshheading:12446687-Transcription Factors,
pubmed-meshheading:12446687-Transcriptional Activation,
pubmed-meshheading:12446687-beta Catenin
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pubmed:year |
2003
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pubmed:articleTitle |
Identification of a promoter-specific transcriptional activation domain at the C terminus of the Wnt effector protein T-cell factor 4.
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pubmed:affiliation |
Max-Planck-Institut für Immunbiologie, Stübeweg 51, D-79108 Freiburg, Germany. hecht@mm11.ukl.uni-freiburg.de
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pubmed:publicationType |
Journal Article
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