| pubmed-article:12438538 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12438538 | lifeskim:mentions | umls-concept:C0009319 | lld:lifeskim |
| pubmed-article:12438538 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:12438538 | lifeskim:mentions | umls-concept:C0032403 | lld:lifeskim |
| pubmed-article:12438538 | lifeskim:mentions | umls-concept:C0079925 | lld:lifeskim |
| pubmed-article:12438538 | pubmed:dateCreated | 2002-11-19 | lld:pubmed |
| pubmed-article:12438538 | pubmed:abstractText | poly(ADP-ribose) polymerase-2 (PARP-2) is a newly described member of the PARP family of nuclear enzymes. Previous studies have shown pharmacological inhibition of PARP activity to have a beneficial role in attenuating inflammation. We developed a chemically modified 2'-O-(2-methoxy)ethyl antisense oligonucleotide (ISIS 110251) inhibitor of PARP-2 and tested it for efficacy in the interleukin (IL)-10-deficient mouse. In tissue culture, ISIS 110251 reduced PARP-2 mRNA expression in a concentration- and sequence-specific manner. In 129 Sv/Ev mice, ISIS 110251 reduced PARP-2 mRNA in liver by 80%. This reduction was dependent upon treatment duration and was independent of the method of delivery. In interleukin-10-deficient mice with established colitis, treatment with ISIS 110251 normalized colonic epithelial barrier and transport function, reduced proinflammatory cytokine secretion and inducible nitric-oxide synthase activity, and attenuated inflammation. Our data demonstrate that selective inhibition of PARP-2 activity results in a marked improvement of colonic inflammatory disease in a mouse model of chronic colitis and a normalization of colonic function. | lld:pubmed |
| pubmed-article:12438538 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12438538 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12438538 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12438538 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12438538 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12438538 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12438538 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12438538 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12438538 | pubmed:author | pubmed-author:MoniaBrettB | lld:pubmed |
| pubmed-article:12438538 | pubmed:author | pubmed-author:JijonHumberto... | lld:pubmed |
| pubmed-article:12438538 | pubmed:author | pubmed-author:PopoffIanI | lld:pubmed |
| pubmed-article:12438538 | pubmed:author | pubmed-author:TaverniniMich... | lld:pubmed |
| pubmed-article:12438538 | pubmed:author | pubmed-author:MaMichaelM | lld:pubmed |
| pubmed-article:12438538 | pubmed:author | pubmed-author:McKayRobR | lld:pubmed |
| pubmed-article:12438538 | pubmed:author | pubmed-author:MadsenKarenK | lld:pubmed |
| pubmed-article:12438538 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12438538 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12438538 | pubmed:pagination | 1145-54 | lld:pubmed |
| pubmed-article:12438538 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:12438538 | pubmed:articleTitle | Antisense oligonucleotides to poly(ADP-ribose) polymerase-2 ameliorate colitis in interleukin-10-deficient mice. | lld:pubmed |
| pubmed-article:12438538 | pubmed:affiliation | Division of Gastroenterology, University of Alberta, Edmonton, Alberta, Canada. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12438538 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12438538 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12438538 | lld:pubmed |
