Source:http://linkedlifedata.com/resource/pubmed/id/12433685
Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
|
pubmed:dateCreated |
2003-2-28
|
pubmed:abstractText |
Posttranslational processing of Ras proteins has attracted considerable interest as a potential target for anticancer drug discovery. Rce1 encodes an endoprotease that facilitates membrane targeting of Ras and other prenylated proteins by releasing the carboxyl-terminal 3 amino acids (ie, the -AAX of the CAAX motif). Homozygous Rce1 mutant embryos (Rce1(-/-)) die late in gestation. To characterize the role of Rce1 in hematopoiesis, we performed adoptive transfers and investigated cells from the recipients. Rce1(-/-) fetal liver cells rescued lethally irradiated recipients and manifested normal long-term repopulating potential in competitive repopulation assays. The recipients of Rce1(-/-) cells developed modest elevations in mature myeloid cells (neutrophils + monocytes), but remained well. Bone marrow cells from mice that received transplants of Rce1(-/-) activated extracellular signal-related kinase (ERK) normally in response to granulocyte-macrophage colony-stimulating factor. These data suggest that pharmacologic inhibitors of Rce1 will have minimal effects on normal hematopoietic cells.
|
pubmed:grant | |
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
AIM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Endopeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/Granulocyte-Macrophage...,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Rce1 protein, mouse
|
pubmed:status |
MEDLINE
|
pubmed:month |
Mar
|
pubmed:issn |
0006-4971
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
15
|
pubmed:volume |
101
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
2250-2
|
pubmed:dateRevised |
2009-11-19
|
pubmed:meshHeading |
pubmed-meshheading:12433685-Adoptive Transfer,
pubmed-meshheading:12433685-Animals,
pubmed-meshheading:12433685-Bone Marrow Cells,
pubmed-meshheading:12433685-Endopeptidases,
pubmed-meshheading:12433685-Enzyme Activation,
pubmed-meshheading:12433685-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:12433685-Hematopoiesis,
pubmed-meshheading:12433685-Hematopoietic Stem Cell Transplantation,
pubmed-meshheading:12433685-Heterozygote,
pubmed-meshheading:12433685-Leukocyte Count,
pubmed-meshheading:12433685-Liver,
pubmed-meshheading:12433685-Mice,
pubmed-meshheading:12433685-Mice, Inbred C57BL,
pubmed-meshheading:12433685-Mice, Knockout,
pubmed-meshheading:12433685-Mitogen-Activated Protein Kinases,
pubmed-meshheading:12433685-Mutation
|
pubmed:year |
2003
|
pubmed:articleTitle |
Hematologic effects of inactivating the Ras processing enzyme Rce1.
|
pubmed:affiliation |
Department of Pediatrics, Gladstone Institute of Cardiovascular Disease, University of California, San Francisco (UCSF), CA 94143, USA.
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
|