Linked Life Data

12427830

Source:http://linkedlifedata.com/resource/pubmed/id/12427830

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
22
pubmed:dateCreated
2002-11-12
pubmed:abstractText
During limbic epileptogenesis in vivo the dentate granule cells (DGCs) exhibit increased expression of brain-derived neurotrophic factor (BDNF), followed by striking morphologic plasticities, namely the formation of basal dendrites and the sprouting of mossy fibers. We hypothesized that increased expression of BDNF intrinsic to DGCs is sufficient to induce these plasticities. To test this hypothesis, we transfected DGCs in rat hippocampal slice cultures with BDNF or nerve growth factor (NGF) via particle-mediated gene transfer, and we visualized the neuronal processes with cotransfected green fluorescent protein. Transfection with BDNF produced significant increases in axonal branch and basal dendrite number relative to NGF or empty vector controls. Structural changes were prevented by the tyrosine kinase inhibitor K252a. Thus increased expression of BDNF within DGCs is sufficient to induce these morphological plasticities, which may represent one mechanism by which BDNF promotes limbic epileptogenesis.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1529-2401
pubmed:author
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
9754-63
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Increased expression of brain-derived neurotrophic factor induces formation of basal dendrites and axonal branching in dentate granule cells in hippocampal explant cultures.
pubmed:affiliation
Department of Medicine (Neurology), Duke University Medical Center, Durham, North Carolina 27710, USA.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't