Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2002-11-27
pubmed:abstractText
Activin A has potent mesoderm-inducing activity in amphibian embryos and induces various mesodermal tissues in vitro from the isolated presumptive ectoderm. By using a sandwich culture method established to examine activin A activity, we previously demonstrated that activin-treated ectoderm can function as both a head and trunk-tail organizer, depending on the concentration of activin A. By using activin A and undifferentiated presumptive ectoderm, it is theoretically possible to reproduce embryonic induction. Here, we test this hypothesis by studying the induction of cartilage tissue by using the sandwich-culture method. In the sandwiched explants, the mesenchymal cell condensation expressed type II collagen and cartilage homeoprotein-1 mRNA, and subsequently, cartilage were induced as they are in vivo. goosecoid (gsc) mRNA was prominently expressed in the cartilage in the explants. Xenopus distal-less 4 (X-dll4) mRNA was expressed throughout the explants. In Xenopus embryos, gsc expression is restricted to the cartilage of the lower jaw, and X-dll4 is widely expressed in the ventral head region, including craniofacial cartilage. These finding suggest that the craniofacial cartilage, especially lower jaw cartilage, was induced in the activin-treated sandwiched explants. In addition, a normal developmental pattern was recapitulated at the histological and genetic level. This work also suggests that the craniofacial cartilage-induction pathway is downstream of activin A. This study presents a model system suitable for the in vitro analysis of craniofacial cartilage induction in vertebrates.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/12424341-10343391, http://linkedlifedata.com/resource/pubmed/commentcorrection/12424341-10476908, http://linkedlifedata.com/resource/pubmed/commentcorrection/12424341-10791308, http://linkedlifedata.com/resource/pubmed/commentcorrection/12424341-10840693, http://linkedlifedata.com/resource/pubmed/commentcorrection/12424341-10874164, http://linkedlifedata.com/resource/pubmed/commentcorrection/12424341-10973558, http://linkedlifedata.com/resource/pubmed/commentcorrection/12424341-11087627, http://linkedlifedata.com/resource/pubmed/commentcorrection/12424341-11291857, http://linkedlifedata.com/resource/pubmed/commentcorrection/12424341-11307165, http://linkedlifedata.com/resource/pubmed/commentcorrection/12424341-11401526, http://linkedlifedata.com/resource/pubmed/commentcorrection/12424341-1352187, http://linkedlifedata.com/resource/pubmed/commentcorrection/12424341-1619277, http://linkedlifedata.com/resource/pubmed/commentcorrection/12424341-1677215, http://linkedlifedata.com/resource/pubmed/commentcorrection/12424341-1684739, http://linkedlifedata.com/resource/pubmed/commentcorrection/12424341-1801866, http://linkedlifedata.com/resource/pubmed/commentcorrection/12424341-1862079, http://linkedlifedata.com/resource/pubmed/commentcorrection/12424341-1918153, http://linkedlifedata.com/resource/pubmed/commentcorrection/12424341-2221676, http://linkedlifedata.com/resource/pubmed/commentcorrection/12424341-2440339, http://linkedlifedata.com/resource/pubmed/commentcorrection/12424341-7590232, http://linkedlifedata.com/resource/pubmed/commentcorrection/12424341-7690966, http://linkedlifedata.com/resource/pubmed/commentcorrection/12424341-7885473, http://linkedlifedata.com/resource/pubmed/commentcorrection/12424341-7885474, http://linkedlifedata.com/resource/pubmed/commentcorrection/12424341-7893605, http://linkedlifedata.com/resource/pubmed/commentcorrection/12424341-8192239, http://linkedlifedata.com/resource/pubmed/commentcorrection/12424341-8590829, http://linkedlifedata.com/resource/pubmed/commentcorrection/12424341-8855272, http://linkedlifedata.com/resource/pubmed/commentcorrection/12424341-8877444, http://linkedlifedata.com/resource/pubmed/commentcorrection/12424341-8901191, http://linkedlifedata.com/resource/pubmed/commentcorrection/12424341-9013932
pubmed:language
eng
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Activins, http://linkedlifedata.com/resource/pubmed/chemical/Collagen Type II, http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary, http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Goosecoid Protein, http://linkedlifedata.com/resource/pubmed/chemical/Homeodomain Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Inhibin-beta Subunits, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger, http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors, http://linkedlifedata.com/resource/pubmed/chemical/Xenopus Proteins, http://linkedlifedata.com/resource/pubmed/chemical/activin A
pubmed:status
MEDLINE
pubmed:author
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
15474-9
pubmed:dateRevised
2009-11-18
pubmed:articleTitle
Activin A induces craniofacial cartilage from undifferentiated Xenopus ectoderm in vitro.
pubmed:affiliation
Department of Biochemistry and Molecular Biology, Kanagawa Dental College, Yokosuka 238-8580, Japan.