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rdf:type |
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lifeskim:mentions |
umls-concept:C0004611,
umls-concept:C0012854,
umls-concept:C0018894,
umls-concept:C0020964,
umls-concept:C0020971,
umls-concept:C0080202,
umls-concept:C0178719,
umls-concept:C0205171,
umls-concept:C0205263,
umls-concept:C0205369,
umls-concept:C0282580,
umls-concept:C0599894,
umls-concept:C0728938,
umls-concept:C1515655
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pubmed:issue |
1
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pubmed:dateCreated |
2002-11-8
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pubmed:abstractText |
We evaluated here the effect of the intracellular targeting of a helper T-cell (Th) epitope, literiolysin O 215-226 derived from Listeria monocytogenes, on induction of a specific Th by gene gun immunisation. Immunisation of C3H/He mice with pE215LAMP plasmid encoding the Th epitope fused with the endosomal/lysosomal targeting signal of lysosome-associated membrane protein (LAMP)-1 gave the epitope-specific proliferative responses of CD4(+) T lymphocytes. In addition, specific interferon-gamma production from the splenocytes was observed. Concomitantly, pE215LAMP-immunised mice showed moderate, but significant protective immunity against listerial challenge. These results suggest that the intracellular targeting of a Th epitope to endosomal/lysosomal compartments by DNA immunisation is useful for eliciting a specific Th subset in vivo.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Toxins,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes, T-Lymphocyte,
http://linkedlifedata.com/resource/pubmed/chemical/Heat-Shock Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Hemolysin Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Lysosome-Associated Membrane...,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, DNA,
http://linkedlifedata.com/resource/pubmed/chemical/hlyA protein, Listeria monocytogenes
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0378-1097
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
29
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pubmed:volume |
216
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
91-7
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:12423758-Animals,
pubmed-meshheading:12423758-Antigens, CD,
pubmed-meshheading:12423758-Bacterial Toxins,
pubmed-meshheading:12423758-Cell Count,
pubmed-meshheading:12423758-Cells, Cultured,
pubmed-meshheading:12423758-Epitopes, T-Lymphocyte,
pubmed-meshheading:12423758-Heat-Shock Proteins,
pubmed-meshheading:12423758-Hemolysin Proteins,
pubmed-meshheading:12423758-Immunity, Cellular,
pubmed-meshheading:12423758-Immunization,
pubmed-meshheading:12423758-Interferon-gamma,
pubmed-meshheading:12423758-Listeria monocytogenes,
pubmed-meshheading:12423758-Listeriosis,
pubmed-meshheading:12423758-Lysosome-Associated Membrane Glycoproteins,
pubmed-meshheading:12423758-Mice,
pubmed-meshheading:12423758-Mice, Inbred C3H,
pubmed-meshheading:12423758-Spleen,
pubmed-meshheading:12423758-T-Lymphocyte Subsets,
pubmed-meshheading:12423758-T-Lymphocytes, Helper-Inducer,
pubmed-meshheading:12423758-Vaccines, DNA
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pubmed:year |
2002
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pubmed:articleTitle |
Endosomal/lysosomal targeting of a single helper T-cell epitope of an intracellular bacterium by DNA immunisation induces a specific T-cell subset and partial protective immunity in vivo.
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pubmed:affiliation |
Second Department of Internal Medicine, Hamamatsu University School of Medicine, Hamamatsu, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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