Source:http://linkedlifedata.com/resource/pubmed/id/12421963
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
10
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| pubmed:dateCreated |
2002-11-7
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| pubmed:abstractText |
Immunization with recombinant heat shock protein 60 (rHsp60) from Histoplasma capsulatum or a region of the protein designated fragment 3 (F3) confers protection from a subsequent challenge in mice. To determine the T cell repertoire involved in the response to Hsp60, T cell clones from C57BL/6 mice immunized with rHsp60 were generated and examined for Vbeta usage by flow cytometry and RT-PCR. Vbeta8.1/8.2(+) T cells were preferentially expanded; other clones bore Vbeta4, -6, or -11. When Vbeta8.1/8.2(+) cells were depleted in mice, Vbeta4(+) T cell clones were almost exclusively isolated. Measurement of cytokine production demonstrated that nine of 16 Vbeta8.1/8.2(+) clones were Th1, while only three of 13 non-Vbeta8.1/8.2(+) clones were Th1. In mice immunized with rHsp60, depletion of Vbeta8.1/8.2(+), but not Vbeta6(+) plus Vbeta7(+), T cells completely abolished the protective efficacy of Hsp60 to lethal and sublethal challenges. Examination of the TCR revealed that a subset of Vbeta8.1/2(+) clones that produced IFN-gamma and were reactive to F3 shared a common CDR3 sequence, DGGQG. Transfer of these T cell clones into TCR alpha/beta(-/-) or IFN-gamma(-/-) mice significantly improved survival, while transfer of other Vbeta8.1/8.2(+) clones that were F3 reactive but were Th2 or clones that were not reactive to F3 but were Th1 did not confer protection. These data indicate that a distinct subset of Vbeta8.1/8.2(+) T cells is crucial for the generation of a protective response to rHsp60.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Chaperonin 60,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Fungal Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Variable Region,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell...,
http://linkedlifedata.com/resource/pubmed/chemical/T cell receptor peptide Vbeta8.1,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, Synthetic
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| pubmed:status |
MEDLINE
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| pubmed:month |
Nov
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| pubmed:issn |
0022-1767
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
169
|
| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
5818-26
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| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:12421963-Animals,
pubmed-meshheading:12421963-Cell Line,
pubmed-meshheading:12421963-Chaperonin 60,
pubmed-meshheading:12421963-Clone Cells,
pubmed-meshheading:12421963-Cytokines,
pubmed-meshheading:12421963-Fungal Vaccines,
pubmed-meshheading:12421963-Histoplasma,
pubmed-meshheading:12421963-Histoplasmosis,
pubmed-meshheading:12421963-Immunoglobulin Variable Region,
pubmed-meshheading:12421963-Immunotherapy, Adoptive,
pubmed-meshheading:12421963-Lymphocyte Depletion,
pubmed-meshheading:12421963-Male,
pubmed-meshheading:12421963-Mice,
pubmed-meshheading:12421963-Mice, Inbred C57BL,
pubmed-meshheading:12421963-Mice, Knockout,
pubmed-meshheading:12421963-Mice, Nude,
pubmed-meshheading:12421963-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:12421963-T-Lymphocyte Subsets,
pubmed-meshheading:12421963-Vaccines, Synthetic
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| pubmed:year |
2002
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| pubmed:articleTitle |
The protective immune response to heat shock protein 60 of Histoplasma capsulatum is mediated by a subset of V beta 8.1/8.2+ T cells.
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| pubmed:affiliation |
Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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