Linked Life Data

12420144

Source:http://linkedlifedata.com/resource/pubmed/id/12420144

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2002-11-6
pubmed:abstractText
The antifungal protein (AFP) secreted by the mould Aspergillus giganteus is a small, highly basic polypeptide with antifungal activity. Previous work has shown that transcription of the corresponding afp gene is regulated by ambient pH, being suppressed under acidic and strongly induced under alkaline conditions. This observation suggested that the afp gene is regulated by the wide-domain transcriptional factor PacC. Here, we show that two putative PacC binding sites within the afp promoter, denoted afpP1 and afpP2, are efficiently recognised in vitro by a PacC fusion protein of A. nidulans. In addition, we found that phosphate, which was used as a buffering agent during cultivation, plays an important role in regulating afp expression. AFP production was nearly completely inhibited in the presence of external phosphate. The results of Northern analysis indicate that the inhibitory effect of phosphate is mediated at the transcriptional level.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0172-8083
pubmed:author
pubmed:issnType
Print
pubmed:volume
42
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
36-42
pubmed:dateRevised
2005-8-23
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
New insights in the regulation of the afp gene encoding the antifungal protein of Aspergillus giganteus.
pubmed:affiliation
Technische Universität Berlin, Institut für Biotechnologie, Fachgebiet Mikrobiologie und Genetik, Gustav-Meyer-Allee 25, 13355 Berlin, Germany. V.Meyer@LB.TU-Berlin.de
pubmed:publicationType
Journal Article