Source:http://linkedlifedata.com/resource/pubmed/id/12414654
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
21
|
| pubmed:dateCreated |
2002-11-4
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| pubmed:abstractText |
Global gene expression patterns in breast cancer cells after treatment with oxidants (hydrogen peroxide, menadione, and t-butyl hydroperoxide) were investigated in three replicate experiments. RNA collected after treatment (at 1, 3, 7, and 24 h) rather than after a single time point, enabled an analysis of gene expression patterns. Using a 17,000 microarray, template-based clustering and multidimensional scaling analysis of the gene expression over the entire time course identified 421 genes as being either up- or down-regulated by the three oxidants. In contrast, only 127 genes were identified for any single time point and a 2-fold change criteria. Surprisingly, the patterns of gene induction were highly similar among the three oxidants; however, differences were observed, particularly with respect to p53, IL-6, and heat-shock related genes. Replicate experiments increased the statistical confidence of the study, whereas changes in gene expression patterns over a time course demonstrated significant additional information versus a single time point. Analyzing the three oxidants simultaneously by template cluster analysis identified genes that heretofore have not been associated with oxidative stress.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Nov
|
| pubmed:issn |
0008-5472
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| pubmed:author |
pubmed-author:ChandramouliV RVR,
pubmed-author:ChenYidongY,
pubmed-author:ChuangYao-Yu EricYY,
pubmed-author:CoffinDeborahD,
pubmed-author:CookJohn AJA,
pubmed-author:DeGraffWilliamW,
pubmed-author:Gadisetti,
pubmed-author:LiuEdison TET,
pubmed-author:MitchellJames BJB,
pubmed-author:RussoAngeloA,
pubmed-author:TsaiMong-HsunMH,
pubmed-author:YanHailingH,
pubmed-author:ZhaoShupingS
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| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
62
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
6246-54
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:12414654-Breast Neoplasms,
pubmed-meshheading:12414654-Cluster Analysis,
pubmed-meshheading:12414654-Gene Expression,
pubmed-meshheading:12414654-Gene Expression Profiling,
pubmed-meshheading:12414654-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:12414654-Humans,
pubmed-meshheading:12414654-Hydrogen Peroxide,
pubmed-meshheading:12414654-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:12414654-Oxidants,
pubmed-meshheading:12414654-Oxidative Stress,
pubmed-meshheading:12414654-Reproducibility of Results,
pubmed-meshheading:12414654-Transcriptional Activation,
pubmed-meshheading:12414654-Tumor Cells, Cultured,
pubmed-meshheading:12414654-Vitamin K 3,
pubmed-meshheading:12414654-tert-Butylhydroperoxide
|
| pubmed:year |
2002
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| pubmed:articleTitle |
Gene expression after treatment with hydrogen peroxide, menadione, or t-butyl hydroperoxide in breast cancer cells.
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| pubmed:affiliation |
Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892, USA.
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| pubmed:publicationType |
Journal Article
|