Linked Life Data

12413958

Source:http://linkedlifedata.com/resource/pubmed/id/12413958

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2002-11-4
pubmed:abstractText
Histone acetylation is suggested to regulate gene expression in the TCR loci. Using R2CD3 mouse model, we previously showed that germline transcription of the TCRbeta chain gene is discrete between 5' Vbeta regions and Dbeta-Jbeta-Cbeta clusters plus Vbeta14 region. In this study, we investigated a role of histone H3 acetylation in transcriptional regulation of the TCRbeta locus. Our results showed that DN-DP transition is accompanied by significant promotion of histone H3 acetylation in both Dbeta-Jbeta-Cbeta cluster and Vbeta14 region, correlating with up-regulation of germline transcription. Surprisingly, termination of germline transcription of the 5' Vbeta regions was inversely correlated with histone H3 hyperacetylation. Moreover, histone H3 acetylation showed equivalent level in both functionally rearranged Vbeta region with active transcription and unrearranged Vbeta region without transcription in mature T cells. These results suggest that histone acetylation is not a sole limiting factor in both terminating germline Vbeta transcription during DN-DP transition and maintaining functionally rearranged Vbeta gene expression.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
298
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
420-6
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Differential regulation between gene expression and histone H3 acetylation in the variable regions of the TCRbeta locus.
pubmed:affiliation
Department of Immunology, Tokai University School of Medicine, Bouseidai, Isehara, Kanagawa 259-1193, Japan.
pubmed:publicationType
Journal Article