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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2002-11-4
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pubmed:abstractText |
The adherens junctional molecule, vascular endothelial cadherin (VE-cadherin), functions to maintain adherens junction stability and to suppress apoptosis of endothelial cells by forming a complex with vascular endothelial growth factor (VEGF) receptor 2 and members of the armadillo family of cytoplasmic proteins. In order to investigate the dynamics of the association of VE-cadherin with adherens junctions during the initial stages of angiogenesis, human umbilical cord endothelial cells (HUVECs) were stimulated with VEGF to undergo angiogenesis in type-I collagen three-dimensional culture. In confluent monolayers of HUVECs, VE-cadherin and its signaling partner, beta-catenin, as well as the paracellular transmembrane adhesion molecule platelet-endothelial cell adhesion molecule (PECAM-1), were all present in regions of cell-cell contact. Within 3 h of stimulation of angiogenesis, VE-cadherin and beta-catenin were lost from these regions. In contrast, the distribution pattern of PECAM-1 did not alter. After 6 h the majority of endothelial cells had migrated to form a network of capillary cords with cell-cell contacts that contained all three molecules. By metabolic labeling of HUVECs it was found that de novo synthesis of VE-cadherin was not essential for the formation of new adherens junctions. Coimmunoprecipitation and immunoblotting experiments showed that the VE-cadherin and beta-catenin remained associated after they were lost from adherens junctions. Detergent extraction of cells with Triton X-100 indicted that the majority of VE-cadherin and beta-catenin was Triton soluble, indicating that they are only weakly associated with the actin-based cytoskeleton.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD31,
http://linkedlifedata.com/resource/pubmed/chemical/CTNNB1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cadherins,
http://linkedlifedata.com/resource/pubmed/chemical/Catnb protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Collagen Type I,
http://linkedlifedata.com/resource/pubmed/chemical/Cytoskeletal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Endothelial Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Lymphokines,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Vascular Endothelial Growth Factor A,
http://linkedlifedata.com/resource/pubmed/chemical/Vascular Endothelial Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/beta Catenin,
http://linkedlifedata.com/resource/pubmed/chemical/cadherin 5
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0014-4827
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
280
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
159-68
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12413882-Adherens Junctions,
pubmed-meshheading:12413882-Animals,
pubmed-meshheading:12413882-Antigens, CD,
pubmed-meshheading:12413882-Antigens, CD31,
pubmed-meshheading:12413882-Cadherins,
pubmed-meshheading:12413882-Cell Adhesion,
pubmed-meshheading:12413882-Cell Culture Techniques,
pubmed-meshheading:12413882-Cells, Cultured,
pubmed-meshheading:12413882-Collagen Type I,
pubmed-meshheading:12413882-Cytoskeletal Proteins,
pubmed-meshheading:12413882-Endothelial Growth Factors,
pubmed-meshheading:12413882-Endothelium, Vascular,
pubmed-meshheading:12413882-Humans,
pubmed-meshheading:12413882-Immunohistochemistry,
pubmed-meshheading:12413882-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:12413882-Lymphokines,
pubmed-meshheading:12413882-Neovascularization, Physiologic,
pubmed-meshheading:12413882-Rats,
pubmed-meshheading:12413882-Trans-Activators,
pubmed-meshheading:12413882-Vascular Endothelial Growth Factor A,
pubmed-meshheading:12413882-Vascular Endothelial Growth Factors,
pubmed-meshheading:12413882-beta Catenin
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pubmed:year |
2002
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pubmed:articleTitle |
Dynamics of vascular endothelial-cadherin and beta-catenin localization by vascular endothelial growth factor-induced angiogenesis in human umbilical vein cells.
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pubmed:affiliation |
School of Biomedical Sciences, Faculty of Medicine, University of Nottingham, Queens Medical Centre, Nottingham, NG7 2UH, United Kingdom.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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