Linked Life Data

12410808

Source:http://linkedlifedata.com/resource/pubmed/id/12410808

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2002-11-4
pubmed:abstractText
Transformation of plant cells by Agrobacterium tumefaciens involves both bacterial virulence proteins and host proteins. We have previously shown that the Arabidopsis thaliana gene H2A-1 (RAT5), which encodes histone H2A-1, is involved in T-DNA integration into the plant genome. Mutation of RAT5 results in a severely decreased frequency of transformation, whereas overexpression of RAT5 enhances the transformation frequency (Mysore et al., 2000b). We show here that the expression pattern of RAT5 correlates with plant root cells most susceptible to transformation. As opposed to a cyclin-GUS fusion gene whose expression is limited to meristematic tissues, the H2A-1 gene is expressed in many non-dividing cells. Under normal circumstances, the H2A-1 gene is expressed in the elongation zone of the root, the region that is most susceptible to Agrobacterium transformation. In addition, when roots are incubated on medium containing phytohormones, a concomitant shift in H2A-1 expression and transformation susceptibility to the root base is observed. Inoculation of root segments with a transfer-competent, but not a transformation-deficient Agrobacterium strain induces H2A-1 expression. Furthermore, pre-treatment of Arabidopsis root segments with phytohormones both induces H2A-1 expression and increases the frequency of Agrobacterium transformation. Our results suggest that the expression of the H2A-1 gene is both a marker for, and a predictor of, plant cells most susceptible to Agrobacterium transformation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0960-7412
pubmed:author
pubmed:issnType
Print
pubmed:volume
32
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
285-98
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:12410808-Agrobacterium tumefaciens, pubmed-meshheading:12410808-Arabidopsis, pubmed-meshheading:12410808-Arabidopsis Proteins, pubmed-meshheading:12410808-Cyclins, pubmed-meshheading:12410808-DNA, Bacterial, pubmed-meshheading:12410808-DNA, Plant, pubmed-meshheading:12410808-Gene Expression Regulation, Plant, pubmed-meshheading:12410808-Glucuronidase, pubmed-meshheading:12410808-Histones, pubmed-meshheading:12410808-In Situ Hybridization, pubmed-meshheading:12410808-Plant Growth Regulators, pubmed-meshheading:12410808-Plant Roots, pubmed-meshheading:12410808-Plant Tumors, pubmed-meshheading:12410808-Plants, Genetically Modified, pubmed-meshheading:12410808-Promoter Regions, Genetic, pubmed-meshheading:12410808-Recombinant Fusion Proteins, pubmed-meshheading:12410808-Transformation, Genetic
pubmed:year
2002
pubmed:articleTitle
Expression of the Arabidopsis histone H2A-1 gene correlates with susceptibility to Agrobacterium transformation.
pubmed:affiliation
Department of Biological Sciences, Purdue University, West Lafayette, IN 47907-1392, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't