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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
2002-10-31
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pubmed:abstractText |
Arachidonic acid (AA) mainly released from the cell membrane by phospholipase A(2) (PLA(2)) is converted to eicosanoids by the action of cyclooxygenase (COX) and lipoxygenase (LO). In order to find the specific inhibitors of AA metabolism especially PLA(2) and COX-2, 300 plant extracts were evaluated for their inhibitory activity on PGD(2) production from cytokine-induced mouse bone marrow-derived mast cells in vitro. From this screening procedure, the methanol extract of Salvia miltiorrhiza was found to inhibit PGD(2) production and the ethyl acetate subfraction gave the strongest inhibition of five subfractions tested. From this ethyl acetate subfraction, an activity-guided isolation finally gave tanshinone I as an active principle. This investigation deals with the effects of tanshinone I on AA metabolism from lipopolysaccharide (LPS)-induced RAW 264.7 cells and in vivo antiinflammatory activity. Tanshinone I inhibited PGE(2) formation from LPS-induced RAW macrophages (IC(50) = 38 microM). However, this compound did not affect COX-2 activity or COX-2 expression. Tanshinone I was found to be an inhibitor of type IIA human recombinant sPLA(2)(IC(50) = 11 microM) and rabbit recombinant cPLA(2) (IC(50) = 82 microM). In addition, tanshinone I showed in vivo antiinflammatory activity in rat carrageenan-induced paw oedema and adjuvant-induced arthritis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Anti-Inflammatory Agents...,
http://linkedlifedata.com/resource/pubmed/chemical/Arachidonic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Carrageenan,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase 2,
http://linkedlifedata.com/resource/pubmed/chemical/Diterpenes, Abietane,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/PTGS2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Phenanthrenes,
http://linkedlifedata.com/resource/pubmed/chemical/Plant Extracts,
http://linkedlifedata.com/resource/pubmed/chemical/Prostaglandin D2,
http://linkedlifedata.com/resource/pubmed/chemical/Prostaglandin-Endoperoxide Synthases,
http://linkedlifedata.com/resource/pubmed/chemical/tanshinone
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0951-418X
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2002 John Wiley & Sons, Ltd.
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pubmed:issnType |
Print
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pubmed:volume |
16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
616-20
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:12410540-Animals,
pubmed-meshheading:12410540-Anti-Inflammatory Agents, Non-Steroidal,
pubmed-meshheading:12410540-Arachidonic Acid,
pubmed-meshheading:12410540-Arthritis, Experimental,
pubmed-meshheading:12410540-Blotting, Western,
pubmed-meshheading:12410540-Carrageenan,
pubmed-meshheading:12410540-Cyclooxygenase 2,
pubmed-meshheading:12410540-Diterpenes, Abietane,
pubmed-meshheading:12410540-Dose-Response Relationship, Drug,
pubmed-meshheading:12410540-Edema,
pubmed-meshheading:12410540-Humans,
pubmed-meshheading:12410540-Inhibitory Concentration 50,
pubmed-meshheading:12410540-Isoenzymes,
pubmed-meshheading:12410540-Male,
pubmed-meshheading:12410540-Mast Cells,
pubmed-meshheading:12410540-Membrane Proteins,
pubmed-meshheading:12410540-Mice,
pubmed-meshheading:12410540-Phenanthrenes,
pubmed-meshheading:12410540-Phytotherapy,
pubmed-meshheading:12410540-Plant Extracts,
pubmed-meshheading:12410540-Prostaglandin D2,
pubmed-meshheading:12410540-Prostaglandin-Endoperoxide Synthases,
pubmed-meshheading:12410540-Rabbits,
pubmed-meshheading:12410540-Rats,
pubmed-meshheading:12410540-Rats, Sprague-Dawley,
pubmed-meshheading:12410540-Salvia miltiorrhiza
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pubmed:year |
2002
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pubmed:articleTitle |
Effects of tanshinone I isolated from Salvia miltiorrhiza bunge on arachidonic acid metabolism and in vivo inflammatory responses.
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pubmed:affiliation |
College of Pharmacy, Kangwon National University, Chunchon 200-701, Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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