Source:http://linkedlifedata.com/resource/pubmed/id/12409018
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
2002-10-31
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pubmed:databankReference | |
pubmed:abstractText |
An homology-based cloning strategy yielded a full-length cDNA from Artemisia annua that encoded a protein of 60.3 kDa which resembled a sesquiterpene synthase in sequence. Heterologous expression of the gene in Escherichia coli provided a soluble recombinant enzyme capable of catalyzing the divalent metal ion-dependent conversion of farnesyl diphosphate to beta-caryophyllene, a sesquiterpene olefin found in the essential oil of A. annua. In reaction parameters and kinetic properties, beta-caryophyllene synthase resembles other sesquiterpene synthases of angiosperms. The beta-caryophyllene synthase gene is expressed in most plant tissues during early development, and is induced in mature tissue in response to fungal elicitor thus suggesting a role for beta-caryophyllene in plant defense.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Enzymes,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Sesquiterpenes,
http://linkedlifedata.com/resource/pubmed/chemical/caryophyllene
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0031-9422
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
61
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
523-9
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:12409018-Artemisia annua,
pubmed-meshheading:12409018-Cloning, Molecular,
pubmed-meshheading:12409018-DNA, Complementary,
pubmed-meshheading:12409018-Enzyme Induction,
pubmed-meshheading:12409018-Enzymes,
pubmed-meshheading:12409018-Gene Expression Regulation, Plant,
pubmed-meshheading:12409018-Hydrogen-Ion Concentration,
pubmed-meshheading:12409018-Molecular Sequence Data,
pubmed-meshheading:12409018-Molecular Structure,
pubmed-meshheading:12409018-Plant Structures,
pubmed-meshheading:12409018-RNA, Messenger,
pubmed-meshheading:12409018-Sesquiterpenes
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pubmed:year |
2002
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pubmed:articleTitle |
A cDNA clone for beta-caryophyllene synthase from Artemisia annua.
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pubmed:affiliation |
Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, P R, China.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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