Source:http://linkedlifedata.com/resource/pubmed/id/12408025
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
2002-10-31
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pubmed:abstractText |
The subunits of the F0 membrane sector of bovine heart mitochondrial H(+)-ATPase that contact the lipids of the mitochondrial inner membrane were identified with the use of specially synthesized proteoliposomes that contained active mitochondrial H(+)-ATPase and a photoreactive lipid, which was 1-acyl-2-[12-(diazocyclopentadiene-2-carbonylamino)-[12-14C]dodecanoyl]-sn- glycero-3-phosphocholine, 1-acyl-2-[11-([125I]diazoiodocyclopentadiene-2-carbonyloxy)undecanoyl]-sn- glycero-3-phosphocholine, or 1-acyl-2-[12-(diazocyclopentadiene-2-carbonylamino)dodecanoyl]-sn-glycero- 3-phosphocholine, where acyl is a mixture of the residues of palmitic (70%) and stearic (30%) acids. An analysis of the cross-linked products obtained upon the UV-irradiation of these proteoliposomes indicated that subunits c and a of the F0 membrane sector contact the lipids. The cross-linked products were identified by SDS-PAGE and MALDI mass spectrometry.
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pubmed:language |
rus
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Lipids,
http://linkedlifedata.com/resource/pubmed/chemical/Mitochondrial Proton-Translocating...,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Subunits,
http://linkedlifedata.com/resource/pubmed/chemical/Proteolipids,
http://linkedlifedata.com/resource/pubmed/chemical/proteoliposomes
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pubmed:status |
MEDLINE
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pubmed:issn |
0132-3423
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
28
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
411-25
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12408025-Animals,
pubmed-meshheading:12408025-Autoradiography,
pubmed-meshheading:12408025-Cattle,
pubmed-meshheading:12408025-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:12408025-Intracellular Membranes,
pubmed-meshheading:12408025-Membrane Lipids,
pubmed-meshheading:12408025-Mitochondria, Heart,
pubmed-meshheading:12408025-Mitochondrial Proton-Translocating ATPases,
pubmed-meshheading:12408025-Protein Subunits,
pubmed-meshheading:12408025-Proteolipids,
pubmed-meshheading:12408025-Spectrometry, Mass, Matrix-Assisted Laser...
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pubmed:articleTitle |
[Mitochondrial H+-ATPase: identification of subunits of the F0 subcomplex contacting with membrane lipids].
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pubmed:affiliation |
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, GSP Moscow, 117997 Russia.
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pubmed:publicationType |
Journal Article,
English Abstract
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