Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
22
pubmed:dateCreated
2002-10-30
pubmed:abstractText
The objective of the study was to determine the effects and the molecular background of interleukin-1 receptor antagonist (IL-1Ra) and vIL-10 double gene transfer into human synovial fibroblasts from patients with rheumatoid arthritis (RA) using the SCID mouse model for cartilage erosion in RA. RA synovial fibroblasts were transduced with retro- or adenoviruses encoding IL-1Ra and/or viral IL-10 (vIL-10). SCID mice were engrafted subcutaneously with IL-1Ra and vIL-10 transduced human rheumatoid synovial fibroblasts and normal cartilage. In parallel, gene expression analysis before and after gene transfer using RNA arbitrarily primed PCR in combination with cDNA array was performed. vIL-10 and IL-1Ra double gene transfer resulted in inhibition of cartilage invasion and degradation by RA synovial fibroblasts when compared with control transduced and non-transduced implants. Expression of key genes that were altered after double gene transfer were related to the activin pathway. The results demonstrate not only that virus-based gene transfer using a combination of two joint-protective genes is a feasible approach to inhibit cartilage degradation by activated RA synovial fibroblasts, but also that the underlying molecular effects include modulation of the activin pathway.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0969-7128
pubmed:author
pubmed:issnType
Print
pubmed:volume
9
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1508-19
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12407423-Actins, pubmed-meshheading:12407423-Adenoviridae, pubmed-meshheading:12407423-Animals, pubmed-meshheading:12407423-Arthritis, Rheumatoid, pubmed-meshheading:12407423-Cartilage, Articular, pubmed-meshheading:12407423-Female, pubmed-meshheading:12407423-Fibroblasts, pubmed-meshheading:12407423-Gene Expression, pubmed-meshheading:12407423-Gene Therapy, pubmed-meshheading:12407423-Genetic Vectors, pubmed-meshheading:12407423-Humans, pubmed-meshheading:12407423-Interleukin 1 Receptor Antagonist Protein, pubmed-meshheading:12407423-Interleukin-10, pubmed-meshheading:12407423-Mice, pubmed-meshheading:12407423-Mice, SCID, pubmed-meshheading:12407423-Models, Animal, pubmed-meshheading:12407423-Polymerase Chain Reaction, pubmed-meshheading:12407423-Retroviridae, pubmed-meshheading:12407423-Sialoglycoproteins, pubmed-meshheading:12407423-Synovial Membrane, pubmed-meshheading:12407423-Transduction, Genetic, pubmed-meshheading:12407423-Transgenes
pubmed:year
2002
pubmed:articleTitle
Inhibition of cartilage destruction by double gene transfer of IL-1Ra and IL-10 involves the activin pathway.
pubmed:affiliation
Department of Internal Medicine I, University of Regensburg, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't