Source:http://linkedlifedata.com/resource/pubmed/id/12406096
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
2002-10-30
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pubmed:abstractText |
In the present study, we examined the expression of Flk2/Flt3, a tyrosine kinase receptor, on human cord blood CD34+ haematopoietic progenitor/stem cells. In flow cytometric analysis, Flk2/Flt3 was expressed on 80% of CD34+ cells and their immature subpopulations, CD34+CD33- and CD34+CD38- cells. Methycellulose clonal culture of sorted CD34+Flk2/Flt3+ and CD34+Flk2/Flt3- cells showed that most of myelocytic progenitors expressed Flk2/Flt3, but erythroid and haematopoietic multipotential progenitors were shared by both fractions. When 1 x 10(4) lineage marker-negative (Lin-)CD34+Flk2/Flt3- cells were transplanted into non-obese diabetic/severe combined immunodeficient (NOD/SCID) mice, none of the recipients possessed human CD45+ cells in bone marrow 11-12 weeks after the transplantation. In contrast, all recipients transplanted with 1 x 10(4) Lin-CD34+Flk2/Flt3+ cells showed successful engraftment. Furthermore, clonal cells expanded from single Lin-CD34+CD38-Flk2/Flt3+ cells in the culture with Flk2/Flt3 ligand, stem cell factor, thrombopoietin, and a complex of interleukin 6/soluble interleukin 6 receptor were individually transplanted into NOD/SCID mice. At 20 to 21 weeks after the transplantation, three out of 10 clones harvested at d 7 of culture, and three out of six clones at d 14 could reconstitute human haematopoiesis in recipient marrow. These results demonstrated that Flk2/Flt3 was expressed on a wide variety of human haematopoietic cells including long-term-repopulating haematopoietic stem cells.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0007-1048
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pubmed:author |
pubmed-author:AsanoShigetakaS,
pubmed-author:EbiharaYasuhiroY,
pubmed-author:ItoMamoruM,
pubmed-author:ManabeAtsushiA,
pubmed-author:MugishimaHideoH,
pubmed-author:NakahataTatsutoshiT,
pubmed-author:TanakaRyuheiR,
pubmed-author:TsujiKohichiroK,
pubmed-author:UedaTakahiroT,
pubmed-author:WadaMikaM,
pubmed-author:XuMing-JiangMJ
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pubmed:issnType |
Print
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pubmed:volume |
119
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
525-34
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12406096-Animals,
pubmed-meshheading:12406096-Antigens, CD34,
pubmed-meshheading:12406096-Cells, Cultured,
pubmed-meshheading:12406096-Clone Cells,
pubmed-meshheading:12406096-Hematopoiesis,
pubmed-meshheading:12406096-Hematopoietic Stem Cell Transplantation,
pubmed-meshheading:12406096-Hematopoietic Stem Cells,
pubmed-meshheading:12406096-Humans,
pubmed-meshheading:12406096-Membrane Proteins,
pubmed-meshheading:12406096-Mice,
pubmed-meshheading:12406096-Mice, Inbred NOD,
pubmed-meshheading:12406096-Mice, SCID,
pubmed-meshheading:12406096-Transplantation, Heterologous
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pubmed:year |
2002
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pubmed:articleTitle |
Reconstitution of human haematopoiesis in non-obese diabetic/severe combined immunodeficient mice by clonal cells expanded from single CD34+CD38- cells expressing Flk2/Flt3.
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pubmed:affiliation |
Division of Cellular Therapy, Advanced Clinical Research Centre, The Institute of Medical Science, The University of Tokyo, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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