Source:http://linkedlifedata.com/resource/pubmed/id/12402514
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
2002-10-29
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pubmed:abstractText |
This study tests the hypothesis that microtubule (MT) depolymerization facilitates contraction of rat aorta via activation of Rho-kinase. Aortic rings from Sprague-Dawley rats were placed in a muscle bath for the measurement of isometric force generation. Bath temperature was decreased from 37 to 10-20 degrees C (30 min), inducing MT depolymerization. Some vessels were treated with nocodazole (10(-5) M) or colchicine (10(-8)-10(-5) M) to stabilize the MTs in the depolymerized state, and the remaining vessels were treated with dimethyl sulfoxide (DMSO: vehicle). Warming of vessels to 37 degrees C induced a significantly greater contraction in nocodazole- and colchicine-treated vessels as compared with controls, and this increase was blocked by pretreatment with taxol (10(-5) M; a MT stabilizing agent) [force (mg): NOC 1159 +/- 93; COL 1138 +/- 69; DMSO 578 +/- 14; TAX + NOC 526 +/- 43; TAX + COL 538 +/- 90]. Following the sustained contraction in response to rewarming, Rho-kinase inhibition with Y-27632 (10(-5) M) relaxed nocodazole- and colchicine-treated rings to a significantly greater extent as compared to DMSO-treated vessels (percent relaxation: NOC 64 +/- 2; COL 65 +/- 5; DMSO 33 +/- 5). These results support the hypothesis that MT depolymerization facilitates contraction of rat aorta via activation of Rho-kinase.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Colchicine,
http://linkedlifedata.com/resource/pubmed/chemical/Intracellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Nocodazole,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/rho-Associated Kinases
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1537-1891
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
38
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
157-61
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:12402514-Analysis of Variance,
pubmed-meshheading:12402514-Animals,
pubmed-meshheading:12402514-Aorta, Thoracic,
pubmed-meshheading:12402514-Colchicine,
pubmed-meshheading:12402514-Enzyme Activation,
pubmed-meshheading:12402514-Intracellular Signaling Peptides and Proteins,
pubmed-meshheading:12402514-Male,
pubmed-meshheading:12402514-Microtubules,
pubmed-meshheading:12402514-Muscle, Smooth,
pubmed-meshheading:12402514-Muscle Contraction,
pubmed-meshheading:12402514-Nocodazole,
pubmed-meshheading:12402514-Protein-Serine-Threonine Kinases,
pubmed-meshheading:12402514-Rats,
pubmed-meshheading:12402514-Rats, Sprague-Dawley,
pubmed-meshheading:12402514-rho-Associated Kinases
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pubmed:year |
2002
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pubmed:articleTitle |
Microtubule depolymerization facilitates contraction of rat aorta via activation of Rho-kinase.
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pubmed:affiliation |
Department of Physiology, University of Michigan, Ann Arbor, MI 48019, USA. kanchanc@umich.edu
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pubmed:publicationType |
Journal Article,
Comparative Study,
In Vitro,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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