Linked Life Data

12398915

Source:http://linkedlifedata.com/resource/pubmed/id/12398915

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2-3
pubmed:dateCreated
2002-10-25
pubmed:abstractText
We investigated the cardioprotective efficacy of a new compound, 3-[(1H-1-indolyl)methyl] -4-amino 4,5-dihydro-1H,1,2,4 triazole-5-thione (C6458). The effect of C6458 on the reduction of the infarct size and its protective ability against oxidative damage of the myocardium after ischemia-reperfusion was examined in rabbits that were subjected to 30 min regional ischemia and 2 h reperfusion. C6458 was administered by continuous infusion for 30 min starting at the 10th minute of sustained ischemia and ending at the 10th minute of reperfusion (two doses, 100 and 200 micromol/kg BW). Infarct and risk areas were delineated with Zn2+-Cd2+ particles and triphenyl tetrazolium chloride staining. Antioxidant activity was detected spectrophotometrically by the measurement of malondialdehyde formation. C6458 reduced significantly the level of malondialdehyde in rabbits under ischemia-reperfusion at both doses. Interestingly, at the dose of 200 micromol/kg, the compound decreased the malondialdehyde levels from the 1st minute of reperfusion and significantly reduced infarct size. The free radical scavenging properties of the compound were examined in vitro by determination of the interaction with the 1,1-diphenyl-2-picrylhydrazyl (DPPH) stable free radical. The ability of the C6458 to scavenge HO* was established by its competition with dimethyl sulfoxide (DMSO) for HO radicals. The compound tested showed a significant effect in the above assays. We conclude that C6458 possesses a protective effect against both damaged myocardium and infarct size in anesthetized rabbits. This beneficial effect may be attributed, at least in part, to its antioxidant and free radical scavenging activity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0014-2999
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
453
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
271-7
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:12398915-Animals, pubmed-meshheading:12398915-Antioxidants, pubmed-meshheading:12398915-Biphenyl Compounds, pubmed-meshheading:12398915-Chromatography, High Pressure Liquid, pubmed-meshheading:12398915-Dimethyl Sulfoxide, pubmed-meshheading:12398915-Dose-Response Relationship, Drug, pubmed-meshheading:12398915-Free Radical Scavengers, pubmed-meshheading:12398915-Free Radicals, pubmed-meshheading:12398915-Heart Injuries, pubmed-meshheading:12398915-Hydroxyl Radical, pubmed-meshheading:12398915-Indoles, pubmed-meshheading:12398915-Male, pubmed-meshheading:12398915-Malondialdehyde, pubmed-meshheading:12398915-Myocardial Infarction, pubmed-meshheading:12398915-Myocardial Reperfusion Injury, pubmed-meshheading:12398915-Myocardium, pubmed-meshheading:12398915-Picrates, pubmed-meshheading:12398915-Rabbits, pubmed-meshheading:12398915-Triazoles
pubmed:year
2002
pubmed:articleTitle
Reduction of myocardial infarct size in rabbits by a novel indole derivative with antioxidant and free radical scavenging properties.
pubmed:affiliation
Department of Pharmaceutical Chemistry, School of Pharmacy, University of Athens, Panepistimioupolis, Zografou, 15771, Athens, Greece. avmota@yahoo.com
pubmed:publicationType
Journal Article