Linked Life Data

12398187

Source:http://linkedlifedata.com/resource/pubmed/id/12398187

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2002-10-25
pubmed:abstractText
The characterization of global gene expression patterns of microscale samples is important in many areas of biological and clinical research. The choice of carrier is critical for the efficient isolation and successful amplification of RNA at the nanogram level. Here we show that recovery of nanograms of RNA is significantly higher when carrier linear polyacrylamide is supplemented with carrier tRNA. Reverse transcription and in vitro transcription reactions remain efficient and specific in the presence of carrier tRNA. Finally, comparison of GeneChip array hybridization patterns demonstrates that the presence of carrier tRNA does not cause detectable distortion in global amplification. Taken together, tRNA is a superior carrier for the isolation and global amplification of microscale RNA.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0736-6205
pubmed:author
pubmed:issnType
Print
pubmed:volume
33
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
788, 790, 792 passim
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Yeast tRNA as carrier in the isolation of microscale RNA for global amplification and expression profiling.
pubmed:affiliation
Department of Biochemistry, Stanford University School of Medicine, CA 94305-5307, USA. qwa205@stanford.edu
pubmed:publicationType
Research Support, U.S. Gov't, P.H.S., Technical Report