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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
52
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pubmed:dateCreated |
2002-12-23
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pubmed:abstractText |
MRG15 is a novel chromodomain protein that is a member of a family of genes related to MORF4. MORF4 (mortality factor on chromosome 4) induces senescence in a subset of human tumor cell lines. Our previous results indicated that MRG15 (MORF-related gene on chromosome 15) could derepress the B-myb promoter by association with Rb. In this study, sucrose gradient analysis demonstrated that MRG15 was present in two distinct nuclear protein complexes, MAF1 (MRG15-associated factor 1) and MAF2. Rb was associated with MRG15 and PAM14 (a novel coil-coil protein) in MAF1, and a histone acetyl transferase, hMOF, was an MRG15 partner in MAF2. Analysis of deletion mutants of MRG15 indicated that the leucine zipper at the C-terminal region of MRG15 was important for the protein associations in MAF1 and that the N-terminal chromodomain was required for the assembly of the MAF2 protein complex. Consistent with these data was the fact that a histone acetyltransferase activity associated with MRG15 was lost when the chromodomain was deleted and that both mutant MRG15 proteins failed to activate the B-myb promoter. The various mechanisms by which MRG15 could activate gene transcription are discussed.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
27
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pubmed:volume |
277
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
50860-6
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:12397079-Acetyltransferases,
pubmed-meshheading:12397079-Base Sequence,
pubmed-meshheading:12397079-Cell Nucleus,
pubmed-meshheading:12397079-Chromosomal Proteins, Non-Histone,
pubmed-meshheading:12397079-Chromosomes, Human, Pair 15,
pubmed-meshheading:12397079-Cloning, Molecular,
pubmed-meshheading:12397079-Genes, myb,
pubmed-meshheading:12397079-Histone Acetyltransferases,
pubmed-meshheading:12397079-Humans,
pubmed-meshheading:12397079-Molecular Sequence Data,
pubmed-meshheading:12397079-Nucleoproteins,
pubmed-meshheading:12397079-Polymerase Chain Reaction,
pubmed-meshheading:12397079-Promoter Regions, Genetic,
pubmed-meshheading:12397079-Restriction Mapping,
pubmed-meshheading:12397079-Saccharomyces cerevisiae Proteins,
pubmed-meshheading:12397079-Trans-Activators,
pubmed-meshheading:12397079-Transcriptional Activation,
pubmed-meshheading:12397079-Tumor Cells, Cultured
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pubmed:year |
2002
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pubmed:articleTitle |
MRG15, a novel chromodomain protein, is present in two distinct multiprotein complexes involved in transcriptional activation.
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pubmed:affiliation |
Roy M. and Phyllis Gough Huffington Center on Aging, Baylor College of Medicine, Houston, Texas 77030, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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