| pubmed-article:12396721 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12396721 | lifeskim:mentions | umls-concept:C1135183 | lld:lifeskim |
| pubmed-article:12396721 | lifeskim:mentions | umls-concept:C0009015 | lld:lifeskim |
| pubmed-article:12396721 | lifeskim:mentions | umls-concept:C0450429 | lld:lifeskim |
| pubmed-article:12396721 | lifeskim:mentions | umls-concept:C0008633 | lld:lifeskim |
| pubmed-article:12396721 | lifeskim:mentions | umls-concept:C1706290 | lld:lifeskim |
| pubmed-article:12396721 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:12396721 | lifeskim:mentions | umls-concept:C0936012 | lld:lifeskim |
| pubmed-article:12396721 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:12396721 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:12396721 | pubmed:issue | 9 | lld:pubmed |
| pubmed-article:12396721 | pubmed:dateCreated | 2002-10-24 | lld:pubmed |
| pubmed-article:12396721 | pubmed:abstractText | We cloned and sequenced a cDNA that contains the coding sequence of the porcine interleukin-18 receptor alpha chain (PoIL-18Ralpha). Based on the conserved nucleotide sequences between human (HuIL-18Ralpha) and murine IL-18Ralpha (MuIL-18Ralpha), we performed reverse transcription-polymerase chain reaction (RT-PCR) with total RNA prepared from porcine peripheral blood lymphocytes (PBLs) stimulated with PoIL-12 to clone the cDNA of PoIL-18Ralpha. The open reading frame (ORF) of the PoIL-18Ralpha cDNA is 1620 base pairs (bp) in length and encodes 539 amino acids. The predicted amino acid sequence showed 68.2% and 50.2% identity to the human and murine amino acid sequences, respectively. Stimulation with concanavalin A (ConA) and IL-12, but not with IL-4, was shown to upregulate the expression of IL-18Ralpha mRNA in pig PBLs by RT-PCR analysis. Flow cytometric analysis also demonstrated that IL-18Ralpha was constitutively expressed on PoPBLs, and this expression was augmented by ConA stimulation. Furthermore, the PoIL-18Ralpha gene was mapped by fluorescence in situ hybridization (FISH) to porcine chromosome 3 (3q13-q14), near the location at which the IL-1beta gene had already been mapped. The present results will be helpful for understanding PoIL-18 and interferon gamma (IFN-gamma)-mediated T helper 1 (Th1) cell development. | lld:pubmed |
| pubmed-article:12396721 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12396721 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12396721 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12396721 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12396721 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12396721 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12396721 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12396721 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12396721 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12396721 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12396721 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12396721 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12396721 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12396721 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12396721 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12396721 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:12396721 | pubmed:issn | 1079-9907 | lld:pubmed |
| pubmed-article:12396721 | pubmed:author | pubmed-author:MunetaYoshihi... | lld:pubmed |
| pubmed-article:12396721 | pubmed:author | pubmed-author:MoriYasuyukiY | lld:pubmed |
| pubmed-article:12396721 | pubmed:author | pubmed-author:UenishiHirohi... | lld:pubmed |
| pubmed-article:12396721 | pubmed:author | pubmed-author:YasueHiroshiH | lld:pubmed |
| pubmed-article:12396721 | pubmed:author | pubmed-author:AwataTakashiT | lld:pubmed |
| pubmed-article:12396721 | pubmed:author | pubmed-author:YamamotoRyuji... | lld:pubmed |
| pubmed-article:12396721 | pubmed:author | pubmed-author:YoshiharaKazu... | lld:pubmed |
| pubmed-article:12396721 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12396721 | pubmed:volume | 22 | lld:pubmed |
| pubmed-article:12396721 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12396721 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12396721 | pubmed:pagination | 995-1002 | lld:pubmed |
| pubmed-article:12396721 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:12396721 | pubmed:meshHeading | pubmed-meshheading:12396721... | lld:pubmed |
| pubmed-article:12396721 | pubmed:meshHeading | pubmed-meshheading:12396721... | lld:pubmed |
| pubmed-article:12396721 | pubmed:meshHeading | pubmed-meshheading:12396721... | lld:pubmed |
| pubmed-article:12396721 | pubmed:meshHeading | pubmed-meshheading:12396721... | lld:pubmed |
| pubmed-article:12396721 | pubmed:meshHeading | pubmed-meshheading:12396721... | lld:pubmed |
| pubmed-article:12396721 | pubmed:meshHeading | pubmed-meshheading:12396721... | lld:pubmed |
| pubmed-article:12396721 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:12396721 | pubmed:articleTitle | Cloning, expression analyses, and chromosomal location of porcine interleukin-18 receptor alpha chain (IL-18Ralpha). | lld:pubmed |
| pubmed-article:12396721 | pubmed:affiliation | Department of Immunology, National Institute of Animal Health, Tsukuba 305-0856, Japan. ymuneta@affrc.go.jp | lld:pubmed |
| pubmed-article:12396721 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12396721 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:12396721 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:397179 | entrezgene:pubmed | pubmed-article:12396721 | lld:entrezgene |
