Linked Life Data

12396721

Source:http://linkedlifedata.com/resource/pubmed/id/12396721

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2002-10-24
pubmed:abstractText
We cloned and sequenced a cDNA that contains the coding sequence of the porcine interleukin-18 receptor alpha chain (PoIL-18Ralpha). Based on the conserved nucleotide sequences between human (HuIL-18Ralpha) and murine IL-18Ralpha (MuIL-18Ralpha), we performed reverse transcription-polymerase chain reaction (RT-PCR) with total RNA prepared from porcine peripheral blood lymphocytes (PBLs) stimulated with PoIL-12 to clone the cDNA of PoIL-18Ralpha. The open reading frame (ORF) of the PoIL-18Ralpha cDNA is 1620 base pairs (bp) in length and encodes 539 amino acids. The predicted amino acid sequence showed 68.2% and 50.2% identity to the human and murine amino acid sequences, respectively. Stimulation with concanavalin A (ConA) and IL-12, but not with IL-4, was shown to upregulate the expression of IL-18Ralpha mRNA in pig PBLs by RT-PCR analysis. Flow cytometric analysis also demonstrated that IL-18Ralpha was constitutively expressed on PoPBLs, and this expression was augmented by ConA stimulation. Furthermore, the PoIL-18Ralpha gene was mapped by fluorescence in situ hybridization (FISH) to porcine chromosome 3 (3q13-q14), near the location at which the IL-1beta gene had already been mapped. The present results will be helpful for understanding PoIL-18 and interferon gamma (IFN-gamma)-mediated T helper 1 (Th1) cell development.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1079-9907
pubmed:author
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
995-1002
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12396721-Amino Acid Sequence, pubmed-meshheading:12396721-Animals, pubmed-meshheading:12396721-Base Sequence, pubmed-meshheading:12396721-Chromosome Mapping, pubmed-meshheading:12396721-Cloning, Molecular, pubmed-meshheading:12396721-Concanavalin A, pubmed-meshheading:12396721-DNA, Complementary, pubmed-meshheading:12396721-Gene Expression Regulation, pubmed-meshheading:12396721-Humans, pubmed-meshheading:12396721-In Situ Hybridization, Fluorescence, pubmed-meshheading:12396721-Interleukin-12, pubmed-meshheading:12396721-Interleukin-18 Receptor alpha Subunit, pubmed-meshheading:12396721-Interleukin-4, pubmed-meshheading:12396721-Lymphocytes, pubmed-meshheading:12396721-Mice, pubmed-meshheading:12396721-Molecular Sequence Data, pubmed-meshheading:12396721-Open Reading Frames, pubmed-meshheading:12396721-Protein Subunits, pubmed-meshheading:12396721-RNA, Messenger, pubmed-meshheading:12396721-Radiation Hybrid Mapping, pubmed-meshheading:12396721-Receptors, Interleukin, pubmed-meshheading:12396721-Receptors, Interleukin-18, pubmed-meshheading:12396721-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:12396721-Sequence Alignment, pubmed-meshheading:12396721-Sequence Homology, Amino Acid, pubmed-meshheading:12396721-Species Specificity, pubmed-meshheading:12396721-Swine
pubmed:year
2002
pubmed:articleTitle
Cloning, expression analyses, and chromosomal location of porcine interleukin-18 receptor alpha chain (IL-18Ralpha).
pubmed:affiliation
Department of Immunology, National Institute of Animal Health, Tsukuba 305-0856, Japan. ymuneta@affrc.go.jp
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't